PMID- 9477481
OWN - NLM
STAT- MEDLINE
DCOM- 19980326
LR  - 20190831
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 59
IP  - 3-4
DP  - 1997 Nov
TI  - Effect of porcine reproductive and respiratory syndrome virus (PRRSV) (isolate 
      ATCC VR-2385) infection on bactericidal activity of porcine pulmonary 
      intravascular macrophages (PIMs): in vitro comparisons with pulmonary alveolar 
      macrophages (PAMs).
PG  - 323-35
AB  - Porcine pulmonary intravascular macrophages (PIMs) were recovered by in situ 
      pulmonary vascular perfusion with 0.025% collagenase in saline from six 8-week 
      old, crossbred pigs. Pulmonary alveolar macrophages (PAMs) were recovered by 
      bronchoalveolar lavage from the same pigs for comparisons in each assay. The 
      macrophages were exposed to PRRSV (ATCC VR-2385) in vitro for 24 h and infection 
      was confirmed by an indirect immunofluorescence test or transmission electron 
      microscopy. Viral particles tended to accumulate in the vesicles of the Golgi 
      apparatus or endoplasmic reticulum. Bactericidal function assays were performed 
      on the recovered macrophages to determine the effects of the virus on macrophage 
      functions. In vitro PRRSV infection reduced the bactericidal ability of PIMs from 
      68.3% to 56.4% (P < 0.09), and PAMs from 69.3% to 61.0% (P > 0.1) at 24 h 
      post-infection. The mean percentage of bacteria killed by macrophages after PRRSV 
      infection was not significantly different among the treatment groups or between 
      the treatment groups and non-infected controls based on colorimetric MTT 
      bactericidal (Staphylococcus aureus) assay. PRRSV did not affect the ability of 
      PIMs or PAMs to internalize opsonized 125I-iododeoxyuridine-labeled S. aureus (P 
      > 0.05). PRRSV infection significantly decreased the production of superoxide 
      anion (P < 0.01) by 67.0% in PIMs and by 69.4% in PAMs. PRRSV reduced the 
      myeloperoxidase-H2O2-halide product (P < 0.01) by 36.5% for PIMs and by 48.1% for 
      PAMs. The results suggest: (1) PIMs should be considered as an important 
      replication site of PRRSV; (2) PRRSV may have a detrimental effect on both PIMs 
      and PAMs; (3) loss of bactericidal function in PIMs may facilitate hematogenous 
      bacterial infections.
FAU - Thanawongnuwech, R
AU  - Thanawongnuwech R
AD  - Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State 
      University, Ames 50011, USA.
FAU - Thacker, E L
AU  - Thacker EL
FAU - Halbur, P G
AU  - Halbur PG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antigens, Viral)
RN  - 11062-77-4 (Superoxides)
RN  - 9679TC07X4 (Iodine)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - *Blood Bactericidal Activity
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Colorimetry
MH  - Fluorescent Antibody Technique, Indirect
MH  - In Vitro Techniques
MH  - Iodine/metabolism
MH  - Macrophages, Alveolar/*physiology
MH  - Peroxidase/metabolism
MH  - Phagocytosis
MH  - Porcine respiratory and reproductive syndrome virus/*physiology/ultrastructure
MH  - Staphylococcus aureus/*immunology
MH  - Superoxides/metabolism
MH  - Swine
EDAT- 1998/02/27 00:00
MHDA- 1998/02/27 00:01
CRDT- 1998/02/27 00:00
PHST- 1998/02/27 00:00 [pubmed]
PHST- 1998/02/27 00:01 [medline]
PHST- 1998/02/27 00:00 [entrez]
AID - S0165-2427(97)00078-0 [pii]
AID - 10.1016/s0165-2427(97)00078-0 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 1997 Nov;59(3-4):323-35. doi: 
      10.1016/s0165-2427(97)00078-0.