PMID- 9478997
OWN - NLM
STAT- MEDLINE
DCOM- 19980325
LR  - 20211203
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 273
IP  - 9
DP  - 1998 Feb 27
TI  - Isolation and characterization of a novel member of the neural zinc finger 
      factor/myelin transcription factor family with transcriptional repression 
      activity.
PG  - 5366-74
AB  - Myelin transcription factor 1 (MyT1) and neural zinc finger factor 1 (NZF-1) 
      represent the first two members of an emerging family of neural specific, zinc 
      finger-containing DNA-binding proteins. MyT1 has been shown recently to play a 
      critical role in neuronal cell differentiation during development. We have cloned 
      the third member of the NZF/MyT family, referred to as neural zinc finger factor 
      3 (NZF-3). The cDNA sequence predicts a protein of 1,032 amino acids which 
      contains two clusters of zinc fingers similar to MyT1 and NZF-1. Unlike MyT1 and 
      NZF-1, NZF-3 does not contain an acidic domain at the amino terminus or a 
      serine/threonine-rich region between the two finger clusters. NZF-3 binds to a 
      DNA element containing a single copy of the previously described AAAGTTT 
      consensus motif for these factors but exhibits a marked enhancement in relative 
      affinity to a bipartite element containing two copies of the consensus motif. In 
      contrast to MyT1 and NZF-1, which are known to activate transcription, 
      cotransfection experiments revealed that NZF-3 confers repression on the basal 
      activity of promoters containing the consensus binding elements. The 
      identification of an additional member of the NZF/MyT family provides an 
      opportunity to investigate the relative contribution of members of this family of 
      transcription factors to the complex regulatory processes in neural development 
      and homeostasis.
FAU - Yee, K S
AU  - Yee KS
AD  - Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, 
      Republic of Singapore.
FAU - Yu, V C
AU  - Yu VC
LA  - eng
SI  - GENBANK/AF031942
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MYT1 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Myt1l protein, rat)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (ST18 protein, rat)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.1.- (Myt1 kinase, rat)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.1 (PKMYT1 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - *DNA-Binding Proteins
MH  - Gene Expression Regulation
MH  - Humans
MH  - Membrane Proteins
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Polymerase Chain Reaction
MH  - Protein Serine-Threonine Kinases/genetics
MH  - Protein-Tyrosine Kinases/genetics
MH  - Rats
MH  - Repressor Proteins/*genetics/metabolism
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Trans-Activators/genetics
MH  - *Transcription Factors
MH  - Transcription, Genetic
MH  - *Zinc Fingers
EDAT- 1998/03/28 00:00
MHDA- 1998/03/28 00:01
CRDT- 1998/03/28 00:00
PHST- 1998/03/28 00:00 [pubmed]
PHST- 1998/03/28 00:01 [medline]
PHST- 1998/03/28 00:00 [entrez]
AID - S0021-9258(17)47121-3 [pii]
AID - 10.1074/jbc.273.9.5366 [doi]
PST - ppublish
SO  - J Biol Chem. 1998 Feb 27;273(9):5366-74. doi: 10.1074/jbc.273.9.5366.