PMID- 9480830
OWN - NLM
STAT- MEDLINE
DCOM- 19980327
LR  - 20171116
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 243
IP  - 2
DP  - 1998 Feb 13
TI  - Differences in the active site environment of Aspergillus ficuum phytases.
PG  - 458-62
AB  - While Aspergillus ficuum phytaseA (phyA) was rapidly inactivated by 
      1,2-cyclohexanedione and phenylglyoxal, both specific modifiers of arginine, 
      phytaseB (phyB) showed a markedly different behavior. First, phyB was totally 
      insensitive to 1,2-cyclohexanedione even in the presence of 0.2 M guanidinium 
      hydrochloride; second, the enzyme showed a great deal of resistance to 
      inactivation by phenylglyoxal. Taken together, these results indicate that the 
      chemical environment of the active site of phyB is very different from that of 
      the active site of phyA. Despite sequence similarities of the active site region 
      in these two proteins, their differential behavior to arginine modifiers 
      indicates that other parts of the protein play a role in the active site 
      formation. We expected some differences in the structure since the proteins have 
      dissimilar kinetic parameters and pH optima.
FAU - Ullah, A H
AU  - Ullah AH
AD  - Southern Regional Research Center, ARS, USDA, New Orleans, Louisiana 70124, USA.
FAU - Sethumadhavan, K
AU  - Sethumadhavan K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cyclohexanones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fungal Proteins)
RN  - 0 (Isoenzymes)
RN  - 75C1OVW0FJ (1,2-cyclohexanedione)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 3.1.3.26 (6-Phytase)
RN  - JU58VJ6Y3B (Guanidine)
RN  - N45G3015PA (Phenylglyoxal)
SB  - IM
MH  - 6-Phytase/antagonists & inhibitors/*chemistry/metabolism
MH  - Arginine/metabolism
MH  - Aspergillus/*enzymology
MH  - Binding Sites/genetics
MH  - Cyclohexanones/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Fungal Proteins/chemistry/metabolism
MH  - Guanidine/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Isoenzymes/antagonists & inhibitors/metabolism
MH  - Kinetics
MH  - Phenylglyoxal/pharmacology
EDAT- 1998/03/03 00:00
MHDA- 1998/03/03 00:01
CRDT- 1998/03/03 00:00
PHST- 1998/03/03 00:00 [pubmed]
PHST- 1998/03/03 00:01 [medline]
PHST- 1998/03/03 00:00 [entrez]
AID - S0006-291X(98)98117-7 [pii]
AID - 10.1006/bbrc.1998.8117 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1998 Feb 13;243(2):458-62. doi: 
      10.1006/bbrc.1998.8117.