PMID- 9481405
OWN - NLM
STAT- MEDLINE
DCOM- 19980316
LR  - 20171207
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 123
IP  - 2
DP  - 1998 Feb
TI  - Cytokine-mediated differential induction of hepatic activator protein-1 genes.
PG  - 191-8
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) 
      increase the synthesis of hepatic acute-phase proteins; these effects appear 
      mediated by activation of transcription factors. The purpose of this study was to 
      determine the effects of TNF-alpha and IL-6 on expression of the jun family of 
      activator protein-1 (AP-1) transcription factors with the human hepatoma cell 
      line HepG2, a well-characterized model of the hepatic acute-phase response. 
      METHODS: HepG2 cells, treated with either TNF-alpha (100 ng/ml) or IL-6 (10 
      ng/ml), were extracted for RNA and protein (total and nuclear) and analyzed. 
      RESULTS: TNF-alpha increased c-jun and junD mRNA and c-Jun and JunD protein 
      levels, as well as AP-1 binding activity. IL-6 increased c-jun mRNA, c-Jun 
      protein, and AP-1 binding activity but did not affect either junD or junB 
      expression. CONCLUSIONS: TNF-alpha and IL-6 induce a differential pattern of AP-1 
      expression in HepG2 cells; TNF-alpha increases both c-Jun and JunD, whereas IL-6 
      stimulates only c-Jun. Neither TNF-alpha nor IL-6 stimulates JunB. Multiple 
      cytokines, released during stress, may act in concert to stimulate the AP-1 
      proteins, which ultimately culminate in the downstream synthesis of a variety of 
      acute-phase proteins.
FAU - Wang, S
AU  - Wang S
AD  - Department of Surgery, University of Texas Medical Branch, Galveston, 77555-0533, 
      USA.
FAU - Evers, B M
AU  - Evers BM
LA  - eng
GR  - P01 DK35608/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Carcinoma, Hepatocellular/metabolism/pathology
MH  - Cytokines/*physiology
MH  - Gene Expression Regulation/*physiology
MH  - Humans
MH  - Interleukin-6/pharmacology
MH  - Liver/*physiology
MH  - Proto-Oncogene Proteins c-jun/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Transcription Factor AP-1/*genetics/metabolism
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1998/03/03 00:00
MHDA- 1998/03/03 00:01
CRDT- 1998/03/03 00:00
PHST- 1998/03/03 00:00 [pubmed]
PHST- 1998/03/03 00:01 [medline]
PHST- 1998/03/03 00:00 [entrez]
AID - S0039-6060(98)70257-0 [pii]
PST - ppublish
SO  - Surgery. 1998 Feb;123(2):191-8.