PMID- 9481713
OWN - NLM
STAT- MEDLINE
DCOM- 19980319
LR  - 20190512
IS  - 0931-0509 (Print)
IS  - 0931-0509 (Linking)
VI  - 13
IP  - 1
DP  - 1998 Jan
TI  - Platelet-activating factor mediates monocyte chemoattractant protein-1 expression 
      in glomerular immune injury.
PG  - 37-43
AB  - These studies were designed to determine the possible role of platelet-activating 
      factor (PAF) in the production of monocyte chemoattractant protein-1 (MCP-1) in 
      glomerular immune injury. The glomerular lesion was induced in isolated perfused 
      rat kidneys by a rabbit anti-rat-thymocyte serum (ATS) and rat serum (RS) as a 
      complement source. Perfusion of kidneys with ATS and RS results in the selective 
      binding of the antiserum to the glomerular mesangium with consecutive 
      intraglomerular activation of complement. Antibody binding and complement 
      activation induced a significant increase in glomerular MCP-1 mRNA levels when 
      assessed by Northern blotting or RT-PCR. Decomplemented RS or non antibody rabbit 
      IgG had only moderate effects on glomerular MCP-1 mRNA levels. The PAF receptor 
      antagonist WEB 2170 almost completely blocked the ATS and RS induced MCP-1 mRNA 
      levels. Perfusion of control kidneys with PAF increased MCP-1 mRNA expression, an 
      effect which was blocked by WEB 2170. Glomerular MCP-1 protein formation, 
      assessed by Western blotting, was stimulated following ATS and RS and PAF, 
      respectively, was blocked by WEB 2170. These data show that PAF, derived from 
      glomerular resident cells following antibody and complement induced injury, 
      stimulates MCP-1 expression. In addition to the direct effects on leukocyte 
      adhesion and activation PAF may mediate inflammatory cell influx in glomerular 
      injuries due to the release of MCP-1.
FAU - Jocks, T
AU  - Jocks T
AD  - Department of Medicine, University of Hamburg, Germany.
FAU - Freudenberg, J
AU  - Freudenberg J
FAU - Zahner, G
AU  - Zahner G
FAU - Stahl, R A
AU  - Stahl RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Azepines)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Platelet Activating Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Triazoles)
RN  - 9007-36-7 (Complement System Proteins)
RN  - CKS724B66O (bepafant)
SB  - IM
MH  - Animals
MH  - Azepines/pharmacology
MH  - Chemokine CCL2/analysis/*biosynthesis/genetics
MH  - Complement System Proteins/*immunology
MH  - Glomerulonephritis/*metabolism
MH  - Kidney Glomerulus/*metabolism
MH  - Male
MH  - Platelet Activating Factor/*physiology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Rats
MH  - Rats, Wistar
MH  - Triazoles/pharmacology
EDAT- 1998/03/03 00:00
MHDA- 1998/03/03 00:01
CRDT- 1998/03/03 00:00
PHST- 1998/03/03 00:00 [pubmed]
PHST- 1998/03/03 00:01 [medline]
PHST- 1998/03/03 00:00 [entrez]
AID - 10.1093/ndt/13.1.37 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 1998 Jan;13(1):37-43. doi: 10.1093/ndt/13.1.37.