PMID- 9482408
OWN - NLM
STAT- MEDLINE
DCOM- 19980305
LR  - 20190914
IS  - 1077-4114 (Print)
IS  - 1077-4114 (Linking)
VI  - 20
IP  - 1
DP  - 1998 Jan-Feb
TI  - Expression of the c-Myc protein in childhood medulloblastoma.
PG  - 18-25
AB  - PURPOSE: The purpose of this study was to determine the incidence of c-Myc 
      protein expression in medulloblastoma/primitive neuroectodermal tumor (MB/PNET) 
      and to identify mechanisms in addition to c-myc gene amplification that lead to 
      increased protein expression. METHODS: We analyzed c-myc gene copy number, mRNA 
      level and protein expression in a panel of MB/PNET cell lines. C-Myc protein 
      levels were assessed in tumor specimens and cell lines using immunohistochemical 
      staining with a c-Myc-specific monoclonal antibody. RESULTS: Southern analysis 
      confirmed c-myc gene amplification in the D425 MED cell line and re-arrangement 
      of one allele in D283 MED, which was analyzed further and appeared to represent a 
      small deletion 3' of exon 3. C-myc transcript levels were dramatically elevated 
      in both lines. Using a c-myc probe, fluorescence in situ hybridization (FISH) 
      showed c-myc present in 3 tandem copies at 8q24 in D283 MED and multiple copies 
      as double minutes in D425 MED. Immunohistochemistry showed c-Myc protein 
      expression in 9 of 10 tumors and all cell lines, regardless of gene amplification 
      status or level of mRNA expression. CONCLUSIONS: c-Myc protein expression is 
      common in MB/PNET tumor specimens and cell lines. Elevated protein levels are 
      observed in the absence of amplification, suggesting that multiple mechanisms of 
      c-myc dysregulation may be involved in MB/PNET. These studies support a role for 
      c-Myc in the development of this common childhood tumor.
FAU - Bruggers, C S
AU  - Bruggers CS
AD  - Department of Pediatrics, Center for Children at the Huntsman Cancer Institute, 
      University of Utah School of Medicine, Salt Lake City 84112, USA.
FAU - Tai, K F
AU  - Tai KF
FAU - Murdock, T
AU  - Murdock T
FAU - Sivak, L
AU  - Sivak L
FAU - Le, K
AU  - Le K
FAU - Perkins, S L
AU  - Perkins SL
FAU - Coffin, C M
AU  - Coffin CM
FAU - Carroll, W L
AU  - Carroll WL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Hematol Oncol
JT  - Journal of pediatric hematology/oncology
JID - 9505928
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Genes, myc
MH  - Humans
MH  - Medulloblastoma/*chemistry/genetics
MH  - Proto-Oncogene Proteins c-myc/*analysis
MH  - RNA, Messenger/analysis
MH  - Tumor Cells, Cultured
EDAT- 1998/03/03 00:00
MHDA- 1998/03/03 00:01
CRDT- 1998/03/03 00:00
PHST- 1998/03/03 00:00 [pubmed]
PHST- 1998/03/03 00:01 [medline]
PHST- 1998/03/03 00:00 [entrez]
AID - 10.1097/00043426-199801000-00003 [doi]
PST - ppublish
SO  - J Pediatr Hematol Oncol. 1998 Jan-Feb;20(1):18-25. doi: 
      10.1097/00043426-199801000-00003.