PMID- 9482667
OWN - NLM
STAT- MEDLINE
DCOM- 19980330
LR  - 20131121
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 12
IP  - 2
DP  - 1998 Feb
TI  - The transactivation domain AF-2 but not the DNA-binding domain of the estrogen 
      receptor is required to inhibit differentiation of avian erythroid progenitors.
PG  - 263-77
AB  - Earlier work demonstrated that an activated estrogen receptor (ER) is required 
      for long-term self-renewal of c-ErbB-expressing avian erythroid progenitors. 
      Here, we demonstrate that activation of the ER does not only arrest or retard 
      differentiation of early progenitors but that it affects erythroid 
      differentiation at all stages of erythroid maturation. A search for genes whose 
      expression is affected by the ER showed that the 17beta-estradiol-activated 
      receptor suppressed the differentiation-associated up-regulation of Gata-1, 
      SCL-1, and globin genes in partially mature cells. In the same cells, the 
      expression of carbonic anhydrase II (CAII) and histone H5 was enhanced. This led 
      to premature expression of CAII, a possible explanation for the toxic effects of 
      overexpressed ER. Repression specifically required the transactivation domain 
      AF-2, but neither an intact DNA-binding domain (DBD) nor the AF-1 domain. The 
      transcriptional activation of CAII, however, required both an intact AF-2 and a 
      functional DBD. The requirement for the AF-2, but not the DBD, suggested that the 
      ER may compete with other nuclear hormone receptors for transcriptional 
      coactivators that bind AF-2, a domain well conserved within this family of 
      transcription factors. We show, however, that this model does not apply for the 
      most likely candidate, the avian thyroid hormone receptor.
FAU - von Lindern, M
AU  - von Lindern M
AD  - Institute of Molecular Pathology, Vienna Biocenter, Austria. 
      vonlindern@hema.fgg.eur.nl
FAU - Boer, L
AU  - Boer L
FAU - Wessely, O
AU  - Wessely O
FAU - Parker, M
AU  - Parker M
FAU - Beug, H
AU  - Beug H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects/genetics
MH  - Cell Line
MH  - Chickens
MH  - DNA-Binding Proteins/metabolism/*physiology
MH  - Erythroid Precursor Cells/*cytology/metabolism/physiology
MH  - Estradiol/pharmacology
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mice
MH  - Protein Structure, Tertiary
MH  - Receptors, Estrogen/metabolism/*physiology
MH  - Receptors, Thyroid Hormone/physiology
MH  - Transcription, Genetic
MH  - *Transcriptional Activation/drug effects
EDAT- 1998/03/03 00:00
MHDA- 1998/03/03 00:01
CRDT- 1998/03/03 00:00
PHST- 1998/03/03 00:00 [pubmed]
PHST- 1998/03/03 00:01 [medline]
PHST- 1998/03/03 00:00 [entrez]
AID - 10.1210/mend.12.2.0068 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1998 Feb;12(2):263-77. doi: 10.1210/mend.12.2.0068.