PMID- 9483172
OWN - NLM
STAT- MEDLINE
DCOM- 19980304
LR  - 20180216
IS  - 0301-0147 (Print)
IS  - 0301-0147 (Linking)
VI  - 27
IP  - 4
DP  - 1997 Jul-Aug
TI  - Neutralization of DHG, a new depolymerized holothurian glycosaminoglycan, by 
      protamine sulfate and platelet factor 4.
PG  - 174-83
AB  - The neutralization of depolymerized holothurian glycosaminoglycan (DHG), 
      unfractionated heparin (UFH), and low-molecular-weight heparin (LMWH) by 
      protamine sulfate (PS) or platelet factor 4 (PF4) was studied. In in vitro 
      studies, the prolongation of thrombin clotting time (TCT) by these 
      glycosaminoglycans was completely neutralized by PS, whereas activated partial 
      thromboplastin time (APTT) was relatively resistant to neutralization. In rats, 
      prolongation of bleeding time by DHG was neutralized by PS with concomitant 
      normalization of TCT ex vivo. Heparin-cofactor-II-dependent antithrombin activity 
      of DHG or UFH was neutralized by PF4 at a high concentration to the same extent; 
      however, prolongation of the APTT by DHG was more resistant to neutralization by 
      PF4 at a physiological plasma level than that by UFH. In conclusion, since the 
      prolongation of bleeding time by DHG was neutralized by PS with concomitant 
      normalization of TCT, and since PF4 neutralized the antithrombin activity of DHG, 
      these proteins may be useful as antidotes for DHG to prevent bleeding in case of 
      an overdose.
FAU - Sasaki, E
AU  - Sasaki E
AD  - Taiho Pharmaceutical Co., Ltd., Tokushima, Japan.
FAU - Minamiguchi, K
AU  - Minamiguchi K
FAU - Kitazato, K T
AU  - Kitazato KT
FAU - Nagase, H
AU  - Nagase H
FAU - Kitazato, K
AU  - Kitazato K
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Haemostasis
JT  - Haemostasis
JID - 0371574
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Heparin Antagonists)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Protamines)
RN  - 0 (depolymerized holothurian glucosaminoglycan)
RN  - 37270-94-3 (Platelet Factor 4)
RN  - 81604-65-1 (Heparin Cofactor II)
SB  - IM
MH  - Animals
MH  - Bleeding Time
MH  - Drug Interactions
MH  - Glycosaminoglycans/*antagonists & inhibitors
MH  - Heparin Antagonists/pharmacology
MH  - Heparin Cofactor II/antagonists & inhibitors
MH  - Heparin, Low-Molecular-Weight/antagonists & inhibitors
MH  - Humans
MH  - Male
MH  - Partial Thromboplastin Time
MH  - Platelet Factor 4/*pharmacology
MH  - Protamines/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Thrombin Time
EDAT- 1997/07/01 00:00
MHDA- 1998/03/04 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1998/03/04 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1159/000217453 [doi]
PST - ppublish
SO  - Haemostasis. 1997 Jul-Aug;27(4):174-83. doi: 10.1159/000217453.