PMID- 9484774
OWN - NLM
STAT- MEDLINE
DCOM- 19980313
LR  - 20211203
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 16
IP  - 7
DP  - 1998 Feb 19
TI  - Intron-exon structure of the MET gene and cloning of an alternatively-spliced Met 
      isoform reveals frequent exon-skipping of a single large internal exon.
PG  - 833-42
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional factor that 
      stimulates epithelial cell mitogenesis, motility, invasion, and morphogenesis. 
      Its receptor is encoded by the MET proto-oncogene, a transmembrane receptor 
      tyrosine kinase. Several studies have suggested a role for MET as a dominant 
      oncogene in tumor development and progression. Conversely, MET is located at a 
      region on chromosome 7q31 frequently deleted in carcinomas, suggesting that 
      recessive mutations in MET may exist in certain cancers. To facilitate a search 
      for mutations in MET, we have obtained the intron-exon structure of the human MET 
      gene. We present the genomic structure of the first member of the Met receptor 
      family to be characterized. Interestingly, MET contains a large second exon of 
      1214 nucleotides. We show that this exon, containing the AUG for the Met 
      receptor, is frequently skipped in normal human tissues and cell lines, and 
      corresponds to a ubiquitously expressed 7 kb Met transcript. This transcript 
      yields no detectable protein product in vivo. Thus, unlike other genes, in which 
      alternative splicing often gives rise to proteins with distinct activities, 
      exon-skipping of MET exon 2 is predicted to decrease the abundance of a Met mRNA 
      encoding a functional Met receptor.
FAU - Lin, J C
AU  - Lin JC
AD  - Department of Medicine, McGill University, Montreal, Canada.
FAU - Naujokas, M
AU  - Naujokas M
FAU - Zhu, H
AU  - Zhu H
FAU - Nolet, S
AU  - Nolet S
FAU - Park, M
AU  - Park M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Alternative Splicing
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Exons
MH  - Humans
MH  - Introns
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins c-met/*genetics
MH  - *Proto-Oncogenes
MH  - RNA, Messenger/genetics
MH  - Structure-Activity Relationship
EDAT- 1998/03/04 00:00
MHDA- 1998/03/04 00:01
CRDT- 1998/03/04 00:00
PHST- 1998/03/04 00:00 [pubmed]
PHST- 1998/03/04 00:01 [medline]
PHST- 1998/03/04 00:00 [entrez]
AID - 10.1038/sj.onc.1201599 [doi]
PST - ppublish
SO  - Oncogene. 1998 Feb 19;16(7):833-42. doi: 10.1038/sj.onc.1201599.