PMID- 9485079
OWN - NLM
STAT- MEDLINE
DCOM- 19980319
LR  - 20061115
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 41
IP  - 2
DP  - 1998 Feb
TI  - Apoptotic cell clearance in systemic lupus erythematosus. II. Role of 
      beta2-glycoprotein I.
PG  - 215-23
AB  - OBJECTIVE: To analyze the contribution of beta2-glycoprotein I (beta2-GPI) to 
      apoptotic cell recognition by antiphospholipid antibodies (aPL) and macrophages 
      from patients with autoimmune disease. METHODS: Phosphatidylserine expression by 
      Jurkat cells undergoing apoptosis upon CD95 crosslinking or ultraviolet 
      irradiation was verified by confocal microscopy of cells stained with fluorescein 
      isothiocyanate-labeled annexin V. Beta2-GPI was purified by 
      heparin/cationic-exchange chromatography and was biotinylated or used to purify 
      beta2-GPI-specific antibodies by affinity chromatography. Binding to apoptotic 
      cells was assessed by flow cytometry. The clearance of 3H-labeled, apoptotic 
      cells by macrophages was assessed by beta counting. RESULTS: The array of 
      epitopes generated by beta2-GPI association with apoptotic cells specifically 
      targets their recognition and is required for their opsonization by human aPL. 
      Nevertheless, beta2-GPI is not required for apoptotic cell clearance by human 
      macrophages in the absence of aPL. CONCLUSION: The proinflammatory clearance of 
      aPL-opsonized apoptotic cells, but not the nonphlogistic clearance of apoptotic 
      cells by scavenger macrophages, depends on beta2-GPI.
FAU - Manfredi, A A
AU  - Manfredi AA
AD  - Istituto Scientifico H. S. Raffaele, and Universita di Milano, Milan, Italy.
FAU - Rovere, P
AU  - Rovere P
FAU - Heltai, S
AU  - Heltai S
FAU - Galati, G
AU  - Galati G
FAU - Nebbia, G
AU  - Nebbia G
FAU - Tincani, A
AU  - Tincani A
FAU - Balestrieri, G
AU  - Balestrieri G
FAU - Sabbadini, M G
AU  - Sabbadini MG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Antibodies, Antiphospholipid)
RN  - 0 (Glycoproteins)
RN  - 0 (Opsonin Proteins)
RN  - 0 (Phosphatidylserines)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Antibodies, Antiphospholipid/immunology
MH  - Apoptosis/*physiology
MH  - Glycoproteins/metabolism/*physiology
MH  - Humans
MH  - Jurkat Cells
MH  - Lupus Erythematosus, Systemic/metabolism/*pathology/*physiopathology
MH  - Macrophages/physiology
MH  - Opsonin Proteins/immunology
MH  - Phosphatidylserines/metabolism
MH  - beta 2-Glycoprotein I
EDAT- 1998/03/04 00:00
MHDA- 1998/03/04 00:01
CRDT- 1998/03/04 00:00
PHST- 1998/03/04 00:00 [pubmed]
PHST- 1998/03/04 00:01 [medline]
PHST- 1998/03/04 00:00 [entrez]
AID - 10.1002/1529-0131(199802)41:2<215::AID-ART5>3.0.CO;2-X [doi]
PST - ppublish
SO  - Arthritis Rheum. 1998 Feb;41(2):215-23. doi: 
      10.1002/1529-0131(199802)41:2<215::AID-ART5>3.0.CO;2-X.