PMID- 9487995
OWN - NLM
STAT- MEDLINE
DCOM- 19980309
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 155
IP  - 3
DP  - 1997 Dec
TI  - Expression of transforming growth factors alpha and beta-1 messenger RNA in the 
      bovine mammary gland during different stages of development and lactation.
PG  - 501-11
AB  - It is now widely accepted that the mammary gland is under interconnected hormonal 
      and local control. Growth factors are involved in the intercellular signalling of 
      the gland. Our aim was the detection of transforming growth factors alpha 
      (TGF-alpha) and beta 1 (TGF-beta 1) messenger RNA during mammogenesis, 
      lactogenesis, galactopoiesis and involution in the bovine mammary gland (total n 
      = 27). During these stages the RNA was assessed by means of ribonuclease 
      protection assay and reverse transcription-polymerase chain reaction (RT-PCR). To 
      study possible influences of oestrogen, progesterone and prolactin on growth 
      factor expression, mammary RNA was obtained from heifers after induced 
      mammogenesis and lactogenesis, with and without additional prolactin inhibition 
      (total n = 20). Very low levels of TGF-alpha and TGF-beta 1 expression were 
      detected during lactogenesis and galactopoiesis, increasing levels during 
      mammogenesis of primigravid heifers, and highest levels during mammogenesis of 
      virgin heifers and during involution. TGF-alpha expression after induced 
      mammogenesis was greater than after induced lactogenesis or physiological 
      mammogenesis during pregnancy. Furthermore, TGF-alpha mRNA contents increased 
      after prolactin inhibition. TGF-beta 1 expression was almost equal after induced 
      mammogenesis and lactogenesis, but greater than during the physiological 
      mammogenesis and lactogenesis. In conclusion, it can be assumed that growth 
      promoting TGF-alpha and growth inhibiting TGF-beta 1 are co-expressed in the 
      bovine mammary gland. Higher mRNA contents of both factors during mammogenesis 
      and involution may indicate autocrine or paracrine functions for these growth 
      factors during proliferation and reorganisation of the mammary tissue.
FAU - Plath, A
AU  - Plath A
AD  - Institute of Physiology, Tech. Univ. Munich-Weihenstephan, Freising, Germany.
FAU - Einspanier, R
AU  - Einspanier R
FAU - Peters, F
AU  - Peters F
FAU - Sinowatz, F
AU  - Sinowatz F
FAU - Schams, D
AU  - Schams D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Hormone Antagonists)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Transforming Growth Factor beta)
RN  - 3A64E3G5ZO (Bromocriptine)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 76057-06-2 (Transforming Growth Factors)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9002-62-4 (Prolactin)
SB  - IM
MH  - Animals
MH  - Bromocriptine/pharmacology
MH  - Cattle
MH  - Dexamethasone/pharmacology
MH  - Estradiol/pharmacology
MH  - Female
MH  - Hormone Antagonists/pharmacology
MH  - Lactation/*metabolism
MH  - Mammary Glands, Animal/drug effects/*growth & development/*metabolism
MH  - Polymerase Chain Reaction
MH  - Progesterone/pharmacology
MH  - Prolactin/antagonists & inhibitors
MH  - RNA, Messenger/*metabolism
MH  - Transforming Growth Factor alpha/genetics
MH  - Transforming Growth Factor beta/genetics
MH  - Transforming Growth Factors/*genetics
EDAT- 1998/03/06 00:00
MHDA- 1998/03/06 00:01
CRDT- 1998/03/06 00:00
PHST- 1998/03/06 00:00 [pubmed]
PHST- 1998/03/06 00:01 [medline]
PHST- 1998/03/06 00:00 [entrez]
AID - 10.1677/joe.0.1550501 [doi]
PST - ppublish
SO  - J Endocrinol. 1997 Dec;155(3):501-11. doi: 10.1677/joe.0.1550501.