PMID- 9490667
OWN - NLM
STAT- MEDLINE
DCOM- 19980409
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 91
IP  - 6
DP  - 1998 Mar 15
TI  - Human herpesvirus type 8 interleukin-6 homologue is functionally active on human 
      myeloma cells.
PG  - 1858-63
AB  - Seroepidemiology and polymerase chain reaction studies have strongly suggested 
      that human herpesvirus type 8 (HHV-8) is associated with Kaposi's sarcoma, 
      Castleman's disease, and body cavity-based lymphoma. The genome of HHV-8 harbors 
      a viral analogue of the interleukin-6 (IL-6) gene. The amino acid sequence of the 
      viral IL-6 (vIL-6) protein is 24.7% identical to human IL-6 (hIL-6). IL-6 as a 
      B-cell growth and differentiation factor is known to play an essential role in 
      the pathophysiology of B-cell tumors. Thus, it seems possible that virus-encoded 
      IL-6 contributes to malignant growth of HHV-8-positive B-cell lymphatic tumors. 
      We have tested a preparation of HHV-8-derived IL-6 for the ability to promote the 
      proliferation of the human myeloma cell line INA-6, which is strictly dependent 
      on exogenous IL-6 for growth and survival. Viral IL-6 significantly induced DNA 
      synthesis of INA-6 cells, but required much more protein on a weight basis when 
      compared with hIL-6 for maximal proliferation. The proliferative effect of vIL-6 
      was almost completely inhibited by a combination of anti-IL-6 receptor (IL-6R) 
      and anti-gp130 antibodies or IL-6R superantagonist Sant7 and anti-gp130 
      antibodies. This report demonstrates that vIL-6 has proliferative activity on 
      human cells and that the IL-6R and gp130 are involved in vIL-6 signaling in the 
      myeloma cell line INA-6.
FAU - Burger, R
AU  - Burger R
AD  - Division of Hematology/Oncology, Department of Medicine III, the Institute for 
      Clinical and Molecular Virology, University of Erlangen-Nuernberg, Erlangen, 
      Germany.
FAU - Neipel, F
AU  - Neipel F
FAU - Fleckenstein, B
AU  - Fleckenstein B
FAU - Savino, R
AU  - Savino R
FAU - Ciliberto, G
AU  - Ciliberto G
FAU - Kalden, J R
AU  - Kalden JR
FAU - Gramatzki, M
AU  - Gramatzki M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (IL6ST protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Interleukin-6)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Proteins)
RN  - 133483-10-0 (Cytokine Receptor gp130)
SB  - IM
CIN - Blood. 1998 Sep 15;92(6):2186-8. PMID: 9731082
MH  - Antibodies, Monoclonal/pharmacology
MH  - Antigens, CD/immunology/pharmacology
MH  - Cell Division/drug effects
MH  - Cytokine Receptor gp130
MH  - DNA Replication/drug effects
MH  - DNA, Neoplasm/biosynthesis
MH  - Herpesvirus 8, Human/*physiology
MH  - Humans
MH  - Interleukin-6/*pharmacology
MH  - Membrane Glycoproteins/immunology/pharmacology
MH  - Multiple Myeloma/*pathology
MH  - Neoplastic Stem Cells/*drug effects
MH  - Polymerase Chain Reaction
MH  - Receptors, Interleukin-6/antagonists & inhibitors/drug effects/immunology
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Cells, Cultured
MH  - Viral Proteins/*pharmacology
EDAT- 1998/04/16 00:00
MHDA- 1998/04/16 00:01
CRDT- 1998/04/16 00:00
PHST- 1998/04/16 00:00 [pubmed]
PHST- 1998/04/16 00:01 [medline]
PHST- 1998/04/16 00:00 [entrez]
AID - S0006-4971(20)54782-4 [pii]
PST - ppublish
SO  - Blood. 1998 Mar 15;91(6):1858-63.