PMID- 9490779
OWN - NLM
STAT- MEDLINE
DCOM- 19980421
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 26
IP  - 6
DP  - 1998 Mar 15
TI  - Mutational analysis of the RNA pseudoknot involved in efficient ribosomal 
      frameshifting in simian retrovirus-1.
PG  - 1369-72
AB  - Mutational effects on frameshifting efficiency of the RNA pseudoknot involved in 
      ribosomal frameshifting in simian retrovirus-1 (SRV-1) have been investigated. 
      The primary sequence and the proposed secondary structure of the SRV-1 pseudoknot 
      are similar to those of other efficient frameshifting pseudoknots in mouse 
      mammary tumor virus (MMTV) and feline immunodeficiency virus (FIV), where an 
      unpaired adenine nucleotide intercalates between stem 1 and stem 2. In SRV-1 
      pseudoknot, the adenine nucleotide in between stem 1 and stem 2 has a potential 
      to form an A*U base pair with the last uridine nucleotide in the loop 2, 
      resulting in a continuous A-form helix with coaxially stacked stem 1 and stem 2. 
      To test whether this A*U base pairing and coaxial stacking of stem 1 and stem 2 
      is absolutely required for efficient frameshifting in SRV-1, a series of mutants 
      changing this potential A.U base pair to either G.C base pair or A.A, A.G, A.C, 
      G.A, G.G mismatch is generated, and their frameshifting efficiencies are 
      investigated in vitro using rabbit reticulocyte lysate translation assay. The 
      frameshifting abilities of these mutant pseudoknots are similar to that of the 
      wild-type pseudoknot, suggesting that the A*U base pair in between stem 1 and 
      stem 2 is not necessary to promote efficient frameshifting in SRV-1. These 
      results reveal that coaxial stacking of stem 1 and stem 2 with a Watson-Crick A.U 
      base pair in between two stems is not a required structural feature of the 
      pseudoknot for promoting efficient frameshifting in SRV-1. Our mutational data 
      suggest that SRV-1 pseudoknot adopts similar structural features common to other 
      efficient frameshifting pseudoknots as observed in MMTV and FIV.
FAU - Sung, D
AU  - Sung D
AD  - Kumho Life and Environmental Science Laboratory, Korea Kumho Petrochemical Co. 
      Ltd, 572 Ssangam-dong, Kwangsan-ku, Kwangju, Korea.
FAU - Kang, H
AU  - Kang H
LA  - eng
GR  - GM 10840/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Composition
MH  - Base Sequence
MH  - Cats
MH  - *Frameshifting, Ribosomal
MH  - Immunodeficiency Virus, Feline/genetics
MH  - In Vitro Techniques
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - *Mutation
MH  - Nucleic Acid Conformation
MH  - RNA, Viral/*chemistry/*genetics
MH  - Rabbits
MH  - Reticulocytes/metabolism
MH  - Retroviruses, Simian/*genetics
MH  - Species Specificity
PMC - PMC147434
EDAT- 1998/04/29 00:00
MHDA- 1998/04/29 00:01
PMCR- 1998/03/15
CRDT- 1998/04/29 00:00
PHST- 1998/04/29 00:00 [pubmed]
PHST- 1998/04/29 00:01 [medline]
PHST- 1998/04/29 00:00 [entrez]
PHST- 1998/03/15 00:00 [pmc-release]
AID - gkb258 [pii]
AID - 10.1093/nar/26.6.1369 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1998 Mar 15;26(6):1369-72. doi: 10.1093/nar/26.6.1369.