PMID- 9491317
OWN - NLM
STAT- MEDLINE
DCOM- 19980319
LR  - 20071114
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 21
IP  - 2
DP  - 1998 Feb
TI  - Refinement of the commonly deleted segment in myeloid leukemias with a del(20q).
PG  - 75-81
AB  - A deletion of the long arm of chromosome 20 [del(20q)] is a recurring abnormality 
      in a wide spectrum of myeloid disorders. Loss of genetic material from 20q may 
      confer a proliferative advantage to myeloid cells, possibly through loss of 
      function of a tumor suppressor gene. Previously, we analyzed leukemia cells from 
      19 patients with a del(20q) by fluorescence in situ hybridization (FISH) and 
      identified a segment that was deleted in 95% of all patients examined. The 
      deleted interval extended from 20q11.2 to q12, spanned approximately 13 Mb, and 
      was flanked proximally by RPN2 and distally by D20S17. To narrow the commonly 
      deleted segment and facilitate the identification of candidate genes, we have 
      employed molecular approaches in combination with FISH. By using 21 
      microsatellite markers positioned in a recently generated physical map of 20q, we 
      performed allele loss studies in myeloid leukemia cells from 23 patients with a 
      del(20q). The results of these studies allowed us to delineate a new proximal 
      border, flanked by marker D20S206. By FISH analysis of additional leukemia 
      samples from patients with a del(20q), we have also delineated a new distal 
      boundary between markers D20S119 and UT654. As a result of the redesignation of 
      both the proximal and distal boundaries, we have successfully narrowed the 
      commonly deleted segment within 20q12 to a region spanning approximately 8 Mb. 
      Identification of the smallest deleted segment will facilitate the eventual 
      cloning of a candidate myeloid tumor suppressor gene.
FAU - Wang, P W
AU  - Wang PW
AD  - Section of Hematology/Oncology, University of Chicago, Illinois 60637, USA. 
      pwwang@mcis.bsd.uchicago.edu
FAU - Iannantuoni, K
AU  - Iannantuoni K
FAU - Davis, E M
AU  - Davis EM
FAU - Espinosa, R 3rd
AU  - Espinosa R 3rd
FAU - Stoffel, M
AU  - Stoffel M
FAU - Le Beau, M M
AU  - Le Beau MM
LA  - eng
GR  - CA 67021/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Alleles
MH  - *Chromosome Deletion
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Bacterial
MH  - Chromosomes, Human, Pair 20/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid/*genetics
MH  - Microsatellite Repeats
EDAT- 1998/03/10 03:03
MHDA- 2000/06/20 09:00
CRDT- 1998/03/10 03:03
PHST- 1998/03/10 03:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/03/10 03:03 [entrez]
AID - 10.1002/(SICI)1098-2264(199802)21:2<75::AID-GCC1>3.0.CO;2-4 [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1998 Feb;21(2):75-81.