PMID- 9491392
OWN - NLM
STAT- MEDLINE
DCOM- 19980414
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 13
IP  - 1
DP  - 1998 Jan
TI  - Induction of chromosomal aberrations (unstable and stable) by inhibitors of 
      topoisomerase II, m-AMSA and VP16, using conventional Giemsa staining and 
      chromosome painting techniques.
PG  - 39-43
AB  - Frequencies of symmetrical and asymmetrical exchange aberrations induced by two 
      inhibitors of topoisomerase II, namely, 4'-(9-acridinylamino) 
      methanesulfon-m-anisidide (m-AMSA) and etoposide (VP16), were estimated in human 
      peripheral blood lymphocytes. The aberrations were scored using conventional 
      Giemsa staining and fluorescence in situ hybridization (FISH) techniques, using 
      chromosome-specific DNA libraries. Stable aberrations (translocations) were 
      detected using two cocktails of DNA libraries specific for three chromosomes, 
      namely 1, 3 and X and 2, 4 and 8, representing approximately 40% of the whole 
      human genome. The frequencies of dicentrics and translocations increased in a 
      dose-dependent manner, however, m-AMSA was found to be a more potent inducer of 
      chromosomal aberrations in comparison with VP16 (at concentrations at which 
      comparable frequencies of aberrations were induced) by 20- to 30-fold. When 
      corrected for DNA content of chromosomes in each cocktail, a higher frequency of 
      translocations with the cocktail consisting of chromosomes 2, 4 and 8 in 
      comparison with 1, 3 and X was evident. The genomic translocation frequency 
      calculated from chromosome painting analysis for m-AMSA exceeded that estimated 
      for dicentrics by approximately 2-fold. However, for VP16 almost equal 
      frequencies of both types of chromosome exchange were found.
FAU - Mosesso, P
AU  - Mosesso P
AD  - Dipartimento di Agrobiologia e Agrochimica, Universita degli studi della Tuscia, 
      Viterbo, Italy.
FAU - Darroudi, F
AU  - Darroudi F
FAU - van den Berg, M
AU  - van den Berg M
FAU - Vermeulen, S
AU  - Vermeulen S
FAU - Palitti, F
AU  - Palitti F
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Azure Stains)
RN  - 0 (Topoisomerase II Inhibitors)
RN  - 00DPD30SOY (Amsacrine)
RN  - 6PLQ3CP4P3 (Etoposide)
SB  - IM
MH  - Amsacrine/*pharmacology
MH  - *Azure Stains
MH  - Chromosome Aberrations/*genetics
MH  - Chromosome Banding/*methods
MH  - Etoposide/*pharmacology
MH  - Humans
MH  - Male
MH  - Staining and Labeling
MH  - *Topoisomerase II Inhibitors
MH  - Translocation, Genetic/drug effects
EDAT- 1998/03/10 00:00
MHDA- 1998/03/10 00:01
CRDT- 1998/03/10 00:00
PHST- 1998/03/10 00:00 [pubmed]
PHST- 1998/03/10 00:01 [medline]
PHST- 1998/03/10 00:00 [entrez]
AID - 10.1093/mutage/13.1.39 [doi]
PST - ppublish
SO  - Mutagenesis. 1998 Jan;13(1):39-43. doi: 10.1093/mutage/13.1.39.