PMID- 9491914
OWN - NLM
STAT- MEDLINE
DCOM- 19980402
LR  - 20081121
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 14
IP  - 3
DP  - 1998 Feb 10
TI  - Nonsynonymous mutations within the human immunodeficiency virus type 1 p17 gene 
      are clustered to sequences binding to the host human leukocyte antigen class I 
      molecules.
PG  - 241-8
AB  - We have analyzed the relation between intrapatient variabilities of the p17 gene 
      and the location of known host p17 cytotoxic T lymphocyte (CTL) epitopes in five 
      patients infected with human immunodeficiency virus type 1 (HIV-1). All patients 
      were typed with respect to the human leukocyte antigen (HLA) class I type. One to 
      seven previously fine-mapped p17 CTL epitopes corresponded to the HLA class I 
      restriction elements of each patient. An average of 28+/-16% of the p17 gene of 
      each patient encoded CTL epitopes corresponding to the HLA restriction elements 
      of the host. Twenty full-length p17 gene clones were sequenced from each patient. 
      The intrapatient homology between the p17 sequences ranged from 96.4 to 98.9%. 
      The interpatient homology between the consensus sequences of each patient ranged 
      from 83.1 to 91.6%. A total of 246 nucleotide differences within the 100 p17 
      clones was noted. Fifteen (16%) of 96 synonymous substitutions were found within 
      host CTL epitopes, whereas 72 (48%) of 150 nonsynonymous nucleotide changes were 
      found within CTL epitopes corresponding to the HLA restriction elements of the 
      host (p < 0.0001; Fisher's exact test). Subsequently, variable residues 
      indicating the evolution of at least two major p17 species (i.e., >20% of the 
      clones) were determined to be more common at positions contained within these CTL 
      epitopes (p < 0.01). The present data suggest that the evolution of the p17 gene 
      is influenced by contact areas with the host HLA class I molecules.
FAU - Birk, M
AU  - Birk M
AD  - Division of Clinical Virology, Huddinge University Hospital, Sweden.
FAU - Vahlne, A
AU  - Vahlne A
FAU - Sonnerborg, A
AU  - Sonnerborg A
FAU - Sallberg, M
AU  - Sallberg M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Epitopes)
RN  - 0 (Gene Products, gag)
RN  - 0 (HIV Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Viral Proteins)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
RN  - 0 (p17 protein, Human Immunodeficiency Virus Type 1)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Consensus Sequence
MH  - Epitope Mapping
MH  - Epitopes
MH  - Gene Products, gag/chemistry/*genetics
MH  - *Genes, MHC Class I
MH  - Genes, Viral/genetics
MH  - *Genetic Variation
MH  - HIV Antigens/chemistry/*genetics
MH  - HIV Infections/virology
MH  - HIV-1/chemistry/*genetics/*immunology
MH  - Histocompatibility Antigens Class I/genetics/*metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis
MH  - Species Specificity
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - *Viral Proteins
MH  - gag Gene Products, Human Immunodeficiency Virus
EDAT- 1998/03/10 00:00
MHDA- 1998/03/10 00:01
CRDT- 1998/03/10 00:00
PHST- 1998/03/10 00:00 [pubmed]
PHST- 1998/03/10 00:01 [medline]
PHST- 1998/03/10 00:00 [entrez]
AID - 10.1089/aid.1998.14.241 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1998 Feb 10;14(3):241-8. doi: 10.1089/aid.1998.14.241.