PMID- 9494521 OWN - NLM STAT- MEDLINE DCOM- 19980326 LR - 20041117 IS - 0250-7005 (Print) IS - 0250-7005 (Linking) VI - 17 IP - 6D DP - 1997 Nov-Dec TI - Assignment of the human progression associated protein (PAP) to chromosome 12p12.3. PG - 4281-5 AB - Analysis of mammary carcinoma cell lines MCF-7 and MCF-7ADR by differential display resulted in the identification of a new putative transmembrane protein, PAP (Progression Associated Protein). Cell lines MCF-7 and MCF-7ADR represent a model system for the identification of genes involved in the clinical progression of human breast cancer. According to the functions assigned to some members of its family, PAP might be involved in cell cycle regulation and cell-cell interactions. Here we describe the chromosomal localization of the PAP gene by the use of fluorescence in situ hybridization (FISH) with two different genomic DNA probes, derived from the 3'-untranslated and the 5'-translated region of the PAP cDNA sequence. The results of the chromosomal localization experiments indicate that the PAP gene is located on human chromosome 12p12.3. FAU - Schiemann, S AU - Schiemann S AD - Boehringer Mannheim GmbH, Penzberg, Germany. FAU - Valentine, M AU - Valentine M FAU - Weidle, U H AU - Weidle UH LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 SB - IM MH - Breast Neoplasms/*genetics MH - Cell Cycle MH - Chromosome Mapping MH - *Chromosomes, Human, Pair 12 MH - Female MH - Gene Library MH - Humans MH - In Situ Hybridization, Fluorescence MH - Karyotyping MH - Tumor Cells, Cultured EDAT- 1998/03/12 00:00 MHDA- 1998/03/12 00:01 CRDT- 1998/03/12 00:00 PHST- 1998/03/12 00:00 [pubmed] PHST- 1998/03/12 00:01 [medline] PHST- 1998/03/12 00:00 [entrez] PST - ppublish SO - Anticancer Res. 1997 Nov-Dec;17(6D):4281-5.