PMID- 9494617
OWN - NLM
STAT- MEDLINE
DCOM- 19980325
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 101
IP  - 2
DP  - 1998 Mar
TI  - A Philadelphia negative chronic myelogenous leukemia with the chimeric BCR/ABL 
      gene on chromosome 9 and a b3-a2 splice junction.
PG  - 143-7
AB  - Approximately 5% of patients with chronic myelogenous leukemia (CML) do not 
      reveal the Philadelphia (Ph) chromosome cytogenetically and are termed 
      Ph-negative CML cases. We report one such case, which appeared normal by routine 
      banding techniques. The BCR/ABL rearrangement was detected by reverse 
      transcriptase-polymerase chain reaction and Southern blotting analysis, which 
      suggested a b3-a2 splice junction. Dual color fluorescence in situ hybridization 
      (FISH) with BCR and ABL DNA probes showed that the chimeric fusion gene was 
      localized on chromosome 9q34, rather than at the typical location on chromosome 
      22q11. The BCR/ABL rearrangement was detected in 75% of the patient's bone-marrow 
      population, whereas the remaining 25% of the cells appeared normal. The use of 
      dual color FISH in the diagnosis of CML is extremely valuable not only in 
      identifying cases of Ph-negative CML, but also in quantifying the proportion of 
      transformed cell populations. This information ultimately results in an 
      enhancement of our ability to monitor therapy, follow disease progression, and 
      determine transplant eligibility.
FAU - Macera, M J
AU  - Macera MJ
AD  - Long Island College Hospital/SUNY Health Science Center, Brooklyn, NY 11201, USA.
FAU - Smith, L J
AU  - Smith LJ
FAU - Frankel, E
AU  - Frankel E
FAU - Szabo, P
AU  - Szabo P
FAU - Verma, R S
AU  - Verma RS
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Chromosome Aberrations
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 9
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - RNA, Neoplasm/analysis
MH  - Transcription, Genetic
EDAT- 1998/03/12 00:00
MHDA- 1998/03/12 00:01
CRDT- 1998/03/12 00:00
PHST- 1998/03/12 00:00 [pubmed]
PHST- 1998/03/12 00:01 [medline]
PHST- 1998/03/12 00:00 [entrez]
AID - S0165460897002574 [pii]
AID - 10.1016/s0165-4608(97)00257-4 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Mar;101(2):143-7. doi: 
      10.1016/s0165-4608(97)00257-4.