PMID- 9495252
OWN - NLM
STAT- MEDLINE
DCOM- 19980319
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 75
IP  - 5
DP  - 1998 Mar 2
TI  - Identification of exposed epitopes on the envelope glycoproteins of human T-cell 
      lymphotropic virus type I (HTLV-I).
PG  - 804-13
AB  - Several lines of evidence underscore the important role of the humoral response 
      specific for HTLV-I envelope protein in the protection against viral infection. 
      One approach to producing efficient immunogens is to synthesize peptides 
      corresponding to the primary amino-acid sequence of neutralizing epitopes found 
      in the external sub-unit gp46. In this study, we have selected synthetic peptides 
      overlapping the major linear neutralizing determinants described earlier and used 
      them as immunogens in rabbits and mice. All rabbit polyclonal anti-sera raised 
      against peptides recognized epitopes in a denaturated context as well as MAbs 
      raised against the HB peptide (aa287-311). By contrast, synthetic peptides O 
      (aa89-110), HH (aa190-209), T (aa190-212) and HB (aa287-311) have generated 
      antibodies efficiently binding their epitopes in a native context, suggesting 
      that these domains are well exposed both at the heterodimer and at the oligomer 
      surface. None of the antibodies induced by synthetic peptides show in vitro 
      neutralizing properties, even those with a good capacity to bind the native form 
      of HTLV-I envelope proteins.
FAU - Grange, M P
AU  - Grange MP
AD  - INSERM U271, Lyon, France.
FAU - Rosenberg, A R
AU  - Rosenberg AR
FAU - Horal, P
AU  - Horal P
FAU - Desgranges, C
AU  - Desgranges C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (HTLV-I Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Peptides)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Epitope Mapping
MH  - Giant Cells
MH  - HTLV-I Antigens/*immunology
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Humans
MH  - Membrane Glycoproteins/immunology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Peptides/chemistry/immunology
MH  - Precipitin Tests
MH  - Rabbits
MH  - Viral Envelope Proteins/*immunology
EDAT- 1998/03/12 03:03
MHDA- 2000/06/20 09:00
CRDT- 1998/03/12 03:03
PHST- 1998/03/12 03:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/03/12 03:03 [entrez]
AID - 10.1002/(SICI)1097-0215(19980302)75:5<804::AID-IJC22>3.0.CO;2-4 [pii]
AID - 10.1002/(sici)1097-0215(19980302)75:5<804::aid-ijc22>3.0.co;2-4 [doi]
PST - ppublish
SO  - Int J Cancer. 1998 Mar 2;75(5):804-13. doi: 
      10.1002/(sici)1097-0215(19980302)75:5<804::aid-ijc22>3.0.co;2-4.