PMID- 9497502
OWN - NLM
STAT- MEDLINE
DCOM- 19980317
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 92
IP  - 4
DP  - 1997 Dec
TI  - The major dog allergens, Can f 1 and Can f 2, are salivary lipocalin proteins: 
      cloning and immunological characterization of the recombinant forms.
PG  - 577-86
AB  - Canis familiaris allergen 1 (Can f 1) and Canis familiaris allergen 2 (Can f 2) 
      are the two major allergens present in dog dander extracts. We now report the 
      isolation of cDNAs encoding both proteins and present their nucleotide and 
      deduced amino acid sequences. Can f 1, produced by tongue epithelial tissue, has 
      homology with the von Ebner's gland (VEG) protein, a salivary protein not 
      previously thought to have allergenic properties. Can f 2, produced by tongue and 
      parotid gland, has homology with mouse urinary protein (MUP), a known allergen. 
      Both VEG protein and MUP are members of the lipocalin family of small 
      ligand-binding proteins. Recombinant forms of Can f 1 and Can f 2 were produced 
      and tested for immunoglobulin E (IgE) reactivity. Among dog-allergic subjects, 
      45% had IgE directed exclusively to rCan f 1, and 25% had IgE to both rCan f 1 
      and rCan f 2. In addition, both recombinant proteins were able to cross-link IgE 
      and elicit histamine release from peripheral blood leucocytes in vitro. These 
      findings confirm that Can f 1 and Can f 2 are major and minor dog allergens, 
      respectively, and demonstrate that recombinant forms of dog allergens retain at 
      least some IgE-binding epitopes.
FAU - Konieczny, A
AU  - Konieczny A
AD  - ImmuLogic Pharmaceutical Corporation, Waltham MA 02154, USA.
FAU - Morgenstern, J P
AU  - Morgenstern JP
FAU - Bizinkauskas, C B
AU  - Bizinkauskas CB
FAU - Lilley, C H
AU  - Lilley CH
FAU - Brauer, A W
AU  - Brauer AW
FAU - Bond, J F
AU  - Bond JF
FAU - Aalberse, R C
AU  - Aalberse RC
FAU - Wallner, B P
AU  - Wallner BP
FAU - Kasaian, M T
AU  - Kasaian MT
LA  - eng
SI  - GENBANK/AF027177
SI  - GENBANK/AF027178
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (allergen Can f 2)
RN  - 0 (allergen Can f I)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*genetics/immunology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Plant
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - DNA, Complementary/genetics
MH  - Dogs/*immunology
MH  - Histamine Release
MH  - Hypersensitivity/immunology
MH  - Immunoglobulin E/metabolism
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics
MH  - Recombinant Proteins/immunology
MH  - Saliva/*immunology
PMC - PMC1364166
EDAT- 1998/03/14 00:00
MHDA- 1998/03/14 00:01
PMCR- 1998/12/01
CRDT- 1998/03/14 00:00
PHST- 1998/03/14 00:00 [pubmed]
PHST- 1998/03/14 00:01 [medline]
PHST- 1998/03/14 00:00 [entrez]
PHST- 1998/12/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1997.00386.x [doi]
PST - ppublish
SO  - Immunology. 1997 Dec;92(4):577-86. doi: 10.1046/j.1365-2567.1997.00386.x.