PMID- 9499052
OWN - NLM
STAT- MEDLINE
DCOM- 19980312
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 72
IP  - 3
DP  - 1998 Mar
TI  - Nucleocapsid and matrix protein contributions to selective human immunodeficiency 
      virus type 1 genomic RNA packaging.
PG  - 1983-93
AB  - The nucleocapsid protein (NC) of retroviruses plays a major role in genomic RNA 
      packaging, and some evidence has implicated the matrix protein (MA) of certain 
      retroviruses in viral RNA binding. To further investigate the role of NC in the 
      selective recognition of genomic viral RNA and to address the potential 
      contribution of MA in this process, we constructed chimeric and deletion human 
      immunodeficiency virus type 1 (HIV-1) mutants that alter the NC or MA protein. 
      Both HIV and mouse mammary tumor virus (MMTV) NC proteins have two zinc-binding 
      domains and similar basic amino acid compositions but differ substantially in 
      total length, amino acid sequence, and spacing of the zinc-binding motifs. When 
      the entire NC coding sequence of HIV was replaced with the MMTV NC coding 
      sequence, we found that the HIV genome was incorporated into virions at 50% of 
      wild-type levels. Viruses produced from chimeric HIV genomes with complete NC 
      replacements, or with the two NC zinc-binding domains replaced with MMTV 
      sequences, preferentially incorporated HIV genomes when both HIV and MMTV genomes 
      were simultaneously present in the cell. Viruses produced from chimeric MMTV 
      genomes in which the MMTV NC had been replaced with HIV NC preferentially 
      incorporated MMTV genomes when both HIV and MMTV genomes were simultaneously 
      present in the cell. In contrast, viruses produced from chimeric HIV genomes 
      containing the Moloney NC, which contains a single zinc-binding motif, were 
      previously shown to preferentially incorporate Moloney genomic RNA. Taken 
      together, these results indicate that an NC protein with two zinc-binding motifs 
      is required for specific HIV RNA packaging and that the amino acid context of 
      these motifs, while contributing to the process, is less crucial for specificity. 
      The data also suggest that HIV NC may not be the exclusive determinant of RNA 
      selectivity. Analysis of an HIV MA mutant revealed that specific RNA packaging 
      does not require MA protein.
FAU - Poon, D T
AU  - Poon DT
AD  - Department of Medicine, Children's Hospital, Harvard Medical School, Boston, 
      Massachusetts 02115, USA.
FAU - Li, G
AU  - Li G
FAU - Aldovini, A
AU  - Aldovini A
LA  - eng
GR  - AI36060/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Capsid Proteins)
RN  - 0 (Gene Products, gag)
RN  - 0 (HIV Antigens)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (NCP7 protein, Human immunodeficiency virus 1)
RN  - 0 (Protein Precursors)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (Viral Proteins)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
RN  - 0 (p17 protein, Human Immunodeficiency Virus Type 1)
RN  - 0 (p55 gag precursor protein, Human immunodeficiency virus 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Capsid/genetics/*metabolism
MH  - *Capsid Proteins
MH  - Cloning, Molecular
MH  - Gene Products, gag/analysis/genetics/*metabolism
MH  - HIV Antigens/genetics/*metabolism
MH  - HIV Core Protein p24/analysis
MH  - HIV-1/*genetics/*metabolism/physiology
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis
MH  - Nucleocapsid/genetics/metabolism
MH  - Protein Precursors/analysis
MH  - *RNA, Viral
MH  - Tumor Cells, Cultured
MH  - Viral Matrix Proteins/genetics/metabolism
MH  - *Viral Proteins
MH  - Virion/metabolism/ultrastructure
MH  - *Virus Assembly
MH  - *Zinc Fingers
MH  - gag Gene Products, Human Immunodeficiency Virus
PMC - PMC109491
EDAT- 1998/03/14 00:00
MHDA- 1998/03/14 00:01
PMCR- 1998/03/01
CRDT- 1998/03/14 00:00
PHST- 1998/03/14 00:00 [pubmed]
PHST- 1998/03/14 00:01 [medline]
PHST- 1998/03/14 00:00 [entrez]
PHST- 1998/03/01 00:00 [pmc-release]
AID - 1648 [pii]
AID - 10.1128/JVI.72.3.1983-1993.1998 [doi]
PST - ppublish
SO  - J Virol. 1998 Mar;72(3):1983-93. doi: 10.1128/JVI.72.3.1983-1993.1998.