PMID- 9500294
OWN - NLM
STAT- MEDLINE
DCOM- 19980501
LR  - 20191024
IS  - 0341-2040 (Print)
IS  - 0341-2040 (Linking)
VI  - 176
IP  - 2
DP  - 1998
TI  - Elimination of neutrophils by apoptosis during the resolution of acute pulmonary 
      inflammation in rats.
PG  - 89-98
AB  - We evaluated the apoptosis of neutrophils during the resolution of acute 
      pulmonary inflammation induced by exposure to ozone. The inflammatory response 
      was assessed in rat lungs 0, 1, 3, and 7 days after 4-h exposure to air or 2 ppm 
      ozone. Analysis of bronchoalveolar lavage fluid demonstrated significant 
      increases in albumin concentrations on days 0 and 1 and in the number of 
      lavageable neutrophils on days 0, 1, and 3, indicating the presence of acute 
      pulmonary inflammation. These parameters returned to control values on day 7, 
      which suggests that the acute pulmonary inflammation induced by ozone was 
      reversible. On days 1 and 3, but not on day 0, the neutrophils showed morphologic 
      evidence of apoptosis. Based on morphologic analysis, the proportion of apoptotic 
      neutrophils was 23.3 +/- 2.2% on day 1 and 55.7 +/- 3.2% on day 3. Terminal 
      deoxynucleotidyl transferase-mediated dUTP end labeling (TUNEL), in contrast, 
      revealed that the proportion of apoptotic cells was 59.7 +/- 9.1% on day 1 and 
      68.0 +/- 4.3% on day 3. On day 3, light microscopy and electron microscopy 
      demonstrated engulfment of the neutrophils by macrophages. These findings 
      indicate that the apoptosis of neutrophils followed by their engulfment by 
      macrophages contributes to the clearance of neutrophils from the sites of 
      inflammation. Moreover, TUNEL detected apoptotic neutrophils with greater 
      sensitivity compared with morphologic assessment.
FAU - Ishii, Y
AU  - Ishii Y
AD  - Institute of Clinical Medical Sciences, University of Tsukuba, Ibaraki, Japan.
FAU - Hashimoto, K
AU  - Hashimoto K
FAU - Nomura, A
AU  - Nomura A
FAU - Sakamoto, T
AU  - Sakamoto T
FAU - Uchida, Y
AU  - Uchida Y
FAU - Ohtsuka, M
AU  - Ohtsuka M
FAU - Hasegawa, S
AU  - Hasegawa S
FAU - Sagai, M
AU  - Sagai M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lung
JT  - Lung
JID - 7701875
RN  - 66H7ZZK23N (Ozone)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - *Apoptosis
MH  - Macrophages, Alveolar/pathology
MH  - Male
MH  - Neutrophils/*pathology
MH  - Ozone/toxicity
MH  - Pneumonia/chemically induced/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1998/03/21 00:00
MHDA- 1998/03/21 00:01
CRDT- 1998/03/21 00:00
PHST- 1998/03/21 00:00 [pubmed]
PHST- 1998/03/21 00:01 [medline]
PHST- 1998/03/21 00:00 [entrez]
AID - 10.1007/pl00007597 [doi]
PST - ppublish
SO  - Lung. 1998;176(2):89-98. doi: 10.1007/pl00007597.