PMID- 9500456
OWN - NLM
STAT- MEDLINE
DCOM- 19980325
LR  - 20071114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 58
IP  - 5
DP  - 1998 Mar 1
TI  - Highly efficient and consistent gene transfer into dendritic cells utilizing a 
      combination of ultraviolet-irradiated adenovirus and poly(L-lysine) conjugates.
PG  - 956-61
AB  - Dendritic cells (DCs) are capable of presenting tumor-associated antigens and 
      subsequently play an essential role in T-cell activation. The aim of this study 
      was to develop an efficient method for gene transfer into DCs. These genetically 
      transduced DCs can then be used as potent inducers of specific cell-mediated 
      immune response. When compared with physical methods for gene transfer 
      (lipofection and calcium phosphate precipitation), adenovirus (AdV) vectors 
      proved to be highly efficient for gene transfer into DCs. To overcome concomitant 
      AdV gene expression and potential immunogenicity, AdVs were irradiated with UV. 
      The UV dose was optimized to block AdV transcription without altering AdV 
      receptor binding and endocytosis capacity. We subsequently used a polycationic 
      amino acid compound, poly(L-lysine), to conjugate the irradiated AdVs to 
      transgenes. The resulting complexes were found to mediate a highly efficient 
      transfer of transgenes into DCs, without concomitant expression of AdV gene 
      products. Low titers of irradiated AdVs were sufficient for a consistent gene 
      transfer into DCs. This is the first study to demonstrate efficient, consistent, 
      and practical gene transfer using an UV approach to irradiated AdV-poly(L-lysine) 
      conjugates and should be useful for the development of DC-based tumor vaccine 
      therapies.
FAU - Mulders, P
AU  - Mulders P
AD  - Jonsson Comprehensive Cancer Center, Department of Urology, University of 
      California-Los Angeles School of Medicine, 90095, USA.
FAU - Pang, S
AU  - Pang S
FAU - Dannull, J
AU  - Dannull J
FAU - Kaboo, R
AU  - Kaboo R
FAU - Hinkel, A
AU  - Hinkel A
FAU - Michel, K
AU  - Michel K
FAU - Tso, C L
AU  - Tso CL
FAU - Roth, M
AU  - Roth M
FAU - Belldegrun, A
AU  - Belldegrun A
LA  - eng
GR  - CA66022/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 25104-18-1 (Polylysine)
SB  - IM
MH  - Adenoviridae/radiation effects
MH  - Cells, Cultured
MH  - Dendritic Cells/*physiology
MH  - *Gene Transfer Techniques
MH  - *Genetic Vectors
MH  - Humans
MH  - Polylysine
MH  - Ultraviolet Rays
EDAT- 1998/03/21 00:00
MHDA- 1998/03/21 00:01
CRDT- 1998/03/21 00:00
PHST- 1998/03/21 00:00 [pubmed]
PHST- 1998/03/21 00:01 [medline]
PHST- 1998/03/21 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1998 Mar 1;58(5):956-61.