PMID- 9500904
OWN - NLM
STAT- MEDLINE
DCOM- 19980427
LR  - 20191102
IS  - 1090-7807 (Print)
IS  - 1090-7807 (Linking)
VI  - 130
IP  - 2
DP  - 1998 Feb
TI  - HSQC-based methyl group selection via gradients in mutidimensional NMR 
      spectroscopy of proteins.
PG  - 335-40
AB  - A quadruple-quantum filtered HSQC (QQF-HSQC) for the selection of methyl groups 
      which minimizes the line-broadening effects of proton homonuclear couplings is 
      presented. The scheme uses gradients for n-quantum coherence selection and 
      solvent suppression. In contrast to the heteronuclear quadruple-quantum coherence 
      (HQQC) approach, the QQF-HSQC allows for long constant-time (CT) evolution, 
      making use of the generally favorable relaxation properties of methyl groups. The 
      increase in resolution and concomitant gain in sensitivity is discussed in theory 
      and demonstrated in practice on the 14-kDa human nonpancreatic synovial 
      phospholipase A2 (hnps-PLA2). The constant-time version is particularly useful 
      for obtaining high-resolution spectra as demonstrated on hnps-PLA2. The 
      applicability of the CT-QQF-HSQC module in multidimensional experiments is 
      demonstrated using a 3D CT NOESY-QQF-HSQC spectrum of the 31-kDa homodimeric 
      IIAMan domain of the mannose transporter of E. coli.
CI  - Copyright 1998 Academic Press.
FAU - Diercks, T
AU  - Diercks T
AD  - Institut fur Organische Chemie und Biochemie, Technische Universitat Munchen, 
      Lichtenbergstrase 4, 85747 Garching, Germany.
FAU - Schwaiger, M
AU  - Schwaiger M
FAU - Kessler, H
AU  - Kessler H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Magn Reson
JT  - Journal of magnetic resonance (San Diego, Calif. : 1997)
JID - 9707935
RN  - EC 3.1.1.32 (Phospholipases A)
RN  - EC 3.1.1.4 (Phospholipases A2)
SB  - IM
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - *Nuclear Magnetic Resonance, Biomolecular
MH  - Phospholipases A/*chemistry
MH  - Phospholipases A2
MH  - Synovial Fluid/enzymology
EDAT- 1998/03/17 00:00
MHDA- 1998/03/17 00:01
CRDT- 1998/03/17 00:00
PHST- 1998/03/17 00:00 [pubmed]
PHST- 1998/03/17 00:01 [medline]
PHST- 1998/03/17 00:00 [entrez]
AID - S1090780797913215 [pii]
AID - 10.1006/jmre.1997.1321 [doi]
PST - ppublish
SO  - J Magn Reson. 1998 Feb;130(2):335-40. doi: 10.1006/jmre.1997.1321.