PMID- 9502297
OWN - NLM
STAT- MEDLINE
DCOM- 19980508
LR  - 20071114
IS  - 1083-8791 (Print)
IS  - 1083-8791 (Linking)
VI  - 3
IP  - 6
DP  - 1997 Dec
TI  - Polyclonal engraftment after unrelated donor bone marrow and cord blood 
      transplantation.
PG  - 304-9
AB  - Data on a small number of allogeneic sibling donor marrow transplants have 
      indicated that reconstituted hematopoiesis can be oligoclonal or even monoclonal, 
      suggesting that hematopoiesis can be reconstituted from a very small number of 
      stem cells. However, other studies have failed to confirm this observation. 
      Animal data indicate that hematopoiesis is likely to be oligoclonal after 
      transplantation using limiting numbers of hematopoietic stem cells, or in cases 
      of poor engraftment. In this study we investigated clonality of engraftment after 
      unrelated donor (URD) bone marrow (BM) and cord blood transplantation, clinical 
      settings in which human leukocyte antigen (HLA) disparities have been known to 
      impair engraftment and in which stem cell numbers have been limiting. Twenty-four 
      URD transplant recipients (20, BM; 4, cord blood) together with 8 sibling donor 
      marrow, 2 sibling donor peripheral blood stem cells (PBSC), and 1 sibling donor 
      cord blood recipients were studied at 28 days, 100 days, 6 months, and 1 year 
      post-bone marrow transplantation (BMT) using the polymerase chain reaction 
      (PCR)-based human androgen receptor assay (HUMARA) as a marker of clonality. URD 
      marrow and cord blood recipients show polyclonal reconstitution in all cases, and 
      results are similar at early and late time points after BMT. Similar results were 
      seen in sibling donor marrow and cord blood recipients. These data indicate that 
      oligoclonal hematopoiesis is not a frequent event after URD stem cell 
      transplantation, even for cases in which limited numbers of stem cells are 
      infused.
FAU - Davies, S M
AU  - Davies SM
AD  - Department of Pediatrics, University of Minnesota, Minneapolis 55455, USA.
FAU - Radloff, G A
AU  - Radloff GA
FAU - Wagner, J E
AU  - Wagner JE
FAU - McGlennen, R
AU  - McGlennen R
FAU - Kersey, J H
AU  - Kersey JH
FAU - Ramsay, N K
AU  - Ramsay NK
LA  - eng
GR  - N01-HB-47095/HB/NHLBI NIH HHS/United States
GR  - P01-CA 65493-01/CA/NCI NIH HHS/United States
GR  - R01 HL-32987/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biol Blood Marrow Transplant
JT  - Biology of blood and marrow transplantation : journal of the American Society for 
      Blood and Marrow Transplantation
JID - 9600628
RN  - 0 (Receptors, Androgen)
SB  - IM
MH  - Adolescent
MH  - Alleles
MH  - *Bone Marrow Transplantation
MH  - Child
MH  - Child, Preschool
MH  - Clone Cells
MH  - Colony-Forming Units Assay
MH  - Female
MH  - Hematopoiesis
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Receptors, Androgen/analysis/genetics
EDAT- 1998/03/21 00:00
MHDA- 1998/03/21 00:01
CRDT- 1998/03/21 00:00
PHST- 1998/03/21 00:00 [pubmed]
PHST- 1998/03/21 00:01 [medline]
PHST- 1998/03/21 00:00 [entrez]
PST - ppublish
SO  - Biol Blood Marrow Transplant. 1997 Dec;3(6):304-9.