PMID- 9504345
OWN - NLM
STAT- MEDLINE
DCOM- 19980409
LR  - 20190826
IS  - 0020-7519 (Print)
IS  - 0020-7519 (Linking)
VI  - 28
IP  - 1
DP  - 1998 Jan
TI  - Application of iscom antigen preparations in ELISAs for diagnosis of Neospora and 
      Toxoplasma infections.
PG  - 187-93
AB  - Immunostimulating complexes (iscoms) are cage-like structures of about 40 nm 
      composed of Quil A, cholesterol, phospholipids and antigen. Their main area of 
      use has been as adjuvants and carriers of immunogens in vaccines. Iscoms can also 
      be used for selection of surface membrane proteins of micro-organisms for use in 
      immunoassays, thus decreasing the number of internal proteins that might cause 
      problems with non-specific binding and cross-reactivity. Enzyme-linked 
      immunoassays (ELISAs) utilising parasite antigens incorporated into iscoms have 
      been developed for demonstration of antibodies directed to the intracellular 
      coccidian parasites Toxoplasma gondii and Neospora caninum. These iscom ELISAs 
      have proved very reliable, with high sensitivity and specificity. The preparation 
      of T. gondii and N. caninum iscoms is described, and ELISAs based on iscom 
      antigen preparations that have so far been used for diagnosis of protozoal 
      infections are reviewed.
FAU - Bjorkman, C
AU  - Bjorkman C
AD  - Department of Ruminant Medicine and Veterinary Epidemiology, Swedish University 
      of Agricultural Sciences, Uppsala. camilla.bjorkman@idmed.slu.se
FAU - Lunden, A
AU  - Lunden A
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Int J Parasitol
JT  - International journal for parasitology
JID - 0314024
RN  - 0 (Antigens, Protozoan)
RN  - 0 (ISCOMs)
RN  - 0 (Protozoan Vaccines)
SB  - IM
MH  - Animals
MH  - *Antigens, Protozoan
MH  - Coccidiosis/*diagnosis
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Humans
MH  - *ISCOMs
MH  - Neospora/*immunology/ultrastructure
MH  - Protozoan Vaccines
MH  - Serologic Tests
MH  - Toxoplasma/immunology
MH  - Toxoplasmosis/*diagnosis
RF  - 36
EDAT- 1998/03/21 00:00
MHDA- 1998/03/21 00:01
CRDT- 1998/03/21 00:00
PHST- 1998/03/21 00:00 [pubmed]
PHST- 1998/03/21 00:01 [medline]
PHST- 1998/03/21 00:00 [entrez]
AID - S0020751997001744 [pii]
AID - 10.1016/s0020-7519(97)00174-4 [doi]
PST - ppublish
SO  - Int J Parasitol. 1998 Jan;28(1):187-93. doi: 10.1016/s0020-7519(97)00174-4.