PMID- 9505971
OWN - NLM
STAT- MEDLINE
DCOM- 19980402
LR  - 20190815
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 143
IP  - 1
DP  - 1998
TI  - Analysis of genetic information of an insect picorna-like virus, infectious 
      flacherie virus of silkworm: evidence for evolutionary relationships among 
      insect, mammalian and plant picorna(-like) viruses.
PG  - 127-43
AB  - We synthesized the cDNAs of an insect picornavirus, infectious flacherie virus of 
      silkworm (IFV), genomic RNA and inserted it into a bacterial plasmid (pUC119). 
      The 9,650 nucleotides (nts) sequence except for the poly(A) tail was obtained 
      from the cloned cDNAs, and the sequence integrity was confirmed by primer 
      extension and direct RNA sequencing. The sequence has a large open reading frame 
      (ORF) of 9,255 nts (3,085 codons) flanked by the short 5' non-coding region (156 
      nts) and by the rather long 3' non-coding (239 nts). The structural proteins VP3, 
      4, 1 and 2 were located at the N-terminus of the polyprotein in this order and 
      were preceded by a tentative small peptide. Computer analysis identified the 
      sequences similar to the consensus sequences of 2C (helicase?), 3C (protease), 
      and 3D (RNA polymerase) conserved among mammalian and plant picorna(-like) 
      viruses. In addition, the predicted genome organization of IFV was quite similar 
      to those of picornaviruses. Further analyses of the characteristics of the genome 
      structure and a tentative phylogenetic tree constructed on the basis of the amino 
      acid sequence similarity emphasized the evolutionary relationships among the 
      insect and plant viruses.
FAU - Isawa, H
AU  - Isawa H
AD  - Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, 
      Sapporo, Japan.
FAU - Asano, S
AU  - Asano S
FAU - Sahara, K
AU  - Sahara K
FAU - Iizuka, T
AU  - Iizuka T
FAU - Bando, H
AU  - Bando H
LA  - eng
SI  - GENBANK/AB000906
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Proteins)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Bombyx/*virology
MH  - Cloning, Molecular
MH  - Evolution, Molecular
MH  - Genome, Viral
MH  - Hydrolysis
MH  - Insect Viruses/*genetics
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Picornaviridae/*genetics
MH  - Plant Viruses/genetics
MH  - Proteins/chemistry/genetics/metabolism
MH  - Sequence Analysis
MH  - Sequence Homology, Amino Acid
MH  - Viral Structural Proteins/*genetics
EDAT- 1998/03/20 00:00
MHDA- 1998/03/20 00:01
CRDT- 1998/03/20 00:00
PHST- 1998/03/20 00:00 [pubmed]
PHST- 1998/03/20 00:01 [medline]
PHST- 1998/03/20 00:00 [entrez]
AID - 10.1007/s007050050273 [doi]
PST - ppublish
SO  - Arch Virol. 1998;143(1):127-43. doi: 10.1007/s007050050273.