PMID- 9506990
OWN - NLM
STAT- MEDLINE
DCOM- 19980416
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 273
IP  - 12
DP  - 1998 Mar 20
TI  - Human ZFM1 protein is a transcriptional repressor that interacts with the 
      transcription activation domain of stage-specific activator protein.
PG  - 6868-77
AB  - Stage-specific activator protein (SSAP) is the transcription factor responsible 
      for the activation of the sea urchin late H1 gene at the mid-blastula stage of 
      embryogenesis. SSAP contains an extremely potent transcription activation domain 
      that functions 4-5-fold better than VP16 in a variety of mammalian cell lines. We 
      used the two-hybrid screening technique to identify human cDNAs from an HL60 
      cell-derived cDNA library that encode proteins that interact with the 
      transcription activation domain of SSAP. One of these cDNAs encodes ZFM1, a 
      protein previously identified at the locus linked to multiple endocrine neoplasia 
      type 1 (MEN1) and as presplicing factor SF1. Functional assays establish the ZFM1 
      protein as a transcriptional repressor. ZFM1 protein represses Gal4-GQC-mediated 
      transcription, and this activity requires both a repression domain found in the 
      N-terminal 137 amino acids of the protein, as well as a GQC interaction region. 
      The physiological significance of repression mediated by ZFM1 comes from the 
      ability of its specific repression domain to function when fused to Gal4 and 
      tethered to promoters containing Gal4 binding sites. The activity is unique in 
      that activated but not basal transcription levels are affected.
FAU - Zhang, D
AU  - Zhang D
AD  - Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New 
      York 10461, USA.
FAU - Childs, G
AU  - Childs G
LA  - eng
GR  - GM30333/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (GAL4 protein, S cerevisiae)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA Splicing Factors)
RN  - 0 (Repressor Proteins)
RN  - 0 (SF1 protein, S cerevisiae)
RN  - 0 (SF1 protein, human)
RN  - 0 (SSAP protein, sea urchin)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.1.21 (Thymidine Kinase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carrier Proteins/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Fungal Proteins/metabolism
MH  - Genes, Reporter
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - RNA Splicing Factors
MH  - Repressor Proteins/*metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Thymidine Kinase/genetics
MH  - *Transcription Factors
MH  - *Transcriptional Activation
EDAT- 1998/04/18 00:00
MHDA- 1998/04/18 00:01
CRDT- 1998/04/18 00:00
PHST- 1998/04/18 00:00 [pubmed]
PHST- 1998/04/18 00:01 [medline]
PHST- 1998/04/18 00:00 [entrez]
AID - S0021-9258(18)63961-4 [pii]
AID - 10.1074/jbc.273.12.6868 [doi]
PST - ppublish
SO  - J Biol Chem. 1998 Mar 20;273(12):6868-77. doi: 10.1074/jbc.273.12.6868.