PMID- 9507146
OWN - NLM
STAT- MEDLINE
DCOM- 19980413
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 783
IP  - 2
DP  - 1998 Feb 9
TI  - Distribution and properties of kainate receptors distinct in the CA3 region of 
      the hippocampus of the guinea pig.
PG  - 227-35
AB  - To characterize the nature of kainate (KA) receptors distinct in the CA3 region 
      of the hippocampus, properties of depolarizations induced by pulses of KA or AMPA 
      (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate) applied to dendrites of 
      CA3 neurons with micropipettes were studied in thin transverse slices of the 
      guinea pig hippocampus. KA induced depolarizations at negligible latencies only 
      when administered to the most proximal dendritic areas. The depolarization was 
      unaffected by tetrodotoxin or by a decrease in Ca2+ and an increase in Mg2+ 
      concentrations. The declining slope of the KA-induced depolarization was 
      significantly slower than that of the AMPA-induced depolarization. In comparison 
      with the AMPA-induced depolarization, the KA-induced depolarization was much less 
      susceptible to antagonists such as 6-cyano-7-nitroquinoxaline-2, 3-dione (CNQX) 
      and 1-(4-aminophenyl)-4-methyl-7, 8-methylenedioxy-5H-2,3-benzodiazepine 
      hydrochloride (GYKI52466). 6, 
      7,8,9-Tetrahydro-5-nitro-1H-benz[g]indole-2,3-dione-3-oxime (NS-102) and 
      (2S,4R)-4-methylglutamate (SYM 2081) were without effects. The threshold 
      concentration of pressure-ejected KA to induce depolarizations was about 200 nM. 
      Excitatory postsynaptic potentials elicited by mossy fiber stimulation were more 
      potently suppressed by CNQX than by GYKI52466. These results indicate that 
      receptors responsible for the slow KA depolarization in the CA3 region of the 
      hippocampus are not AMPA receptors but KA receptors. They are localized in the 
      most proximal part of the apical dendrite and distinct from those observed in 
      primary cultures of hippocampal neurons.
FAU - Yamamoto, C
AU  - Yamamoto C
AD  - Department of Physiology, Faculty of Medicine, Kanazawa University, Kanazawa 920, 
      Japan.
FAU - Sawada, S
AU  - Sawada S
FAU - Ohno-Shosaku, T
AU  - Ohno-Shosaku T
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Anti-Anxiety Agents)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Quinoxalines)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, Kainic Acid)
RN  - 102771-26-6 (GYKI 52466)
RN  - 118876-58-7 (2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline)
RN  - 12794-10-4 (Benzodiazepines)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione/pharmacology
MH  - Action Potentials/drug effects
MH  - Animals
MH  - *Anti-Anxiety Agents
MH  - Benzodiazepines/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects
MH  - Guinea Pigs
MH  - Hippocampus/*chemistry/drug effects
MH  - Kainic Acid/pharmacology
MH  - Microinjections
MH  - Pressure
MH  - Quinoxalines/pharmacology
MH  - Receptors, AMPA/analysis/physiology
MH  - Receptors, Kainic Acid/*analysis/*physiology
MH  - Time Factors
EDAT- 1998/04/18 00:00
MHDA- 1998/04/18 00:01
CRDT- 1998/04/18 00:00
PHST- 1998/04/18 00:00 [pubmed]
PHST- 1998/04/18 00:01 [medline]
PHST- 1998/04/18 00:00 [entrez]
AID - S0006-8993(97)01350-4 [pii]
AID - 10.1016/s0006-8993(97)01350-4 [doi]
PST - ppublish
SO  - Brain Res. 1998 Feb 9;783(2):227-35. doi: 10.1016/s0006-8993(97)01350-4.