PMID- 9509688
OWN - NLM
STAT- MEDLINE
DCOM- 19980402
LR  - 20171116
IS  - 0578-1337 (Print)
IS  - 0578-1337 (Linking)
VI  - 61
IP  - 1
DP  - 1998 Jan
TI  - Sperm head agglutination induced by V3 peptide does not occur through a CD4 
      receptor.
PG  - 24-8
AB  - BACKGROUND: Although recent studies have shown that HIV (human immunodeficiency 
      virus) can attach to sperm, the mechanism by which it does this is not yet 
      understood. It has been shown that CD4 receptors on T4 cells are responsible for 
      the binding of gp120 (glycoprotein 120) to HIV; however, the existence of CD4 
      receptors on sperm is controversial. The V3 peptide is part of gp120 and crucial 
      for the syncytium formation by CD4 receptors. In this study we used an anti-CD4 
      antibody to block V3 peptide-induced sperm head agglutination in an attempt to 
      gain a further understanding of the mechanism of HIV attachment to sperm. 
      METHODS: Ten semen samples from 10 healthy men were studied. A sperm head 
      fixation method (SHFM) was used to evaluate the blocking effect of anti-CD4 
      antibody (Q4120) of V3 peptide-induced sperm head agglutination in 
      phosphate-buffered saline solution. RESULTS: While the sperm swam out of the 
      micropipette, as occurs in SHFM, the V3 peptide induced an average of 53.2 +/- 
      10.8 (mean +/- SEM) head-to-head bound sperm (in the 10 semen samples. The sperm 
      that had been preincubated with anti-CD4 antibody induced an average of 54.1 +/- 
      11.6 head-to-head bound sperm. There was no significant difference found between 
      sperm that had been preincubated or not preincubated with anti-CD4 antibody, in 
      terms of sperm head agglutination. CONCLUSIONS: The anti-CD4 antibody can block 
      the binding of gp120 and CD4 positive T cells in a low concentration, but it did 
      not block V3 peptide-induced sperm head agglutination. Therefore, V3 
      peptide-induced sperm head agglutination may not occur through a CD4 receptor.
FAU - Ou, M C
AU  - Ou MC
AD  - Department of Obstetrics and Gynecology, Taipei Medical College Hospital, Taiwan, 
      ROC.
FAU - Tzeng, C R
AU  - Tzeng CR
FAU - Ng, H T
AU  - Ng HT
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Zhonghua Yi Xue Za Zhi (Taipei)
JT  - Zhonghua yi xue za zhi = Chinese medical journal; Free China ed
JID - 0005327
RN  - 0 (CD4 Antigens)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Agglutination
MH  - CD4 Antigens/*physiology
MH  - HIV Envelope Protein gp120/*physiology
MH  - HIV Infections/transmission
MH  - Humans
MH  - Male
MH  - Peptide Fragments/*physiology
MH  - Sperm Head/*virology
EDAT- 1998/03/24 00:00
MHDA- 1998/03/24 00:01
CRDT- 1998/03/24 00:00
PHST- 1998/03/24 00:00 [pubmed]
PHST- 1998/03/24 00:01 [medline]
PHST- 1998/03/24 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi (Taipei). 1998 Jan;61(1):24-8.