PMID- 9510201
OWN - NLM
STAT- MEDLINE
DCOM- 19980326
LR  - 20061115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 160
IP  - 6
DP  - 1998 Mar 15
TI  - Bi-directional induction of matrix metalloproteinase-9 and tissue inhibitor of 
      matrix metalloproteinase-1 during T lymphoma/endothelial cell contact: 
      implication of ICAM-1.
PG  - 2967-73
AB  - The mechanisms that lead to the expression of matrix metalloproteinases (MMP) and 
      tissue inhibitors of MMP (TIMPs) during the invasive process of normal and 
      transformed T cells remain largely unknown. Since vascular cells form a dynamic 
      tissue capable of responding to local stimuli and activating cells through the 
      expression of cytokine receptors and specific cell adhesion molecules, we 
      hypothesized that the firm adhesion of T lymphoma cells to endothelial cells is a 
      critical event in the local production of MMP and TIMP. In the present work, we 
      show that adhesion of lymphoma cells to endothelial cells induced a transient and 
      reciprocal de novo expression of MMP-9 mRNA and enzymatic activity by both cell 
      types. Up-regulation of MMP-9 in T lymphoma cells was concomitant to that of 
      TIMP-1, and required direct contact with endothelial cells. Induction of MMP-9, 
      but not of TIMP-1, was blocked by anti-LFA-1 and anti-intercellular adhesion 
      molecule-1 Abs, indicating that induction of MMP-9 and TIMP-1 in lymphoma cells 
      required direct, yet distinct, intercellular contact. In contrast, the induction 
      of MMP-9 in endothelial cells by T lymphoma cells did not necessitate direct 
      contact and could be achieved by exposure to IL-1 and TNF, or to the supernatant 
      of T lymphoma cell culture. Together, these results demonstrate that firm 
      adhesion of T lymphoma cells to endothelial cells participates in the production 
      of MMP-9 in both cell types through bi-directional signaling pathways, and 
      identify intercellular adhesion molecule-1/LFA-1 as a key interaction in the 
      up-regulation of MMP-9 in T lymphoma cells.
FAU - Aoudjit, F
AU  - Aoudjit F
AD  - Immunology Research Center, Institut Armand-Frappier, Universite du Quebec, 
      Laval, Canada.
FAU - Potworowski, E F
AU  - Potworowski EF
FAU - St-Pierre, Y
AU  - St-Pierre Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - *Cell Communication
MH  - Coculture Techniques
MH  - Collagenases/biosynthesis/*genetics
MH  - Endothelium, Vascular/*cytology
MH  - *Gene Expression Regulation
MH  - Intercellular Adhesion Molecule-1/*physiology
MH  - Lymphocyte Function-Associated Antigen-1/physiology
MH  - Lymphoma, T-Cell/metabolism
MH  - Matrix Metalloproteinase 9
MH  - Mice
MH  - T-Lymphocytes/*physiology
MH  - Tissue Inhibitor of Metalloproteinase-1/biosynthesis/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1998/03/24 00:00
MHDA- 1998/03/24 00:01
CRDT- 1998/03/24 00:00
PHST- 1998/03/24 00:00 [pubmed]
PHST- 1998/03/24 00:01 [medline]
PHST- 1998/03/24 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1998 Mar 15;160(6):2967-73.