PMID- 9510509
OWN - NLM
STAT- MEDLINE
DCOM- 19980416
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 6
IP  - 1
DP  - 1998 Jan
TI  - Comparative mapping of X chromosomes in vole species of the genus Microtus.
PG  - 41-8
AB  - Comparative mapping of X-linked genes has progressed rapidly since Ohno's 
      prediction that genes on the X chromosome should be conserved as a syntenic group 
      in all mammals. Although several conserved blocks of homology between human and 
      mouse have been discovered, rearrangements within the X chromosome have also been 
      characterized. More recently, some exceptions to Ohno's law have been reported. 
      We have used fluorescence in situ hybridization (FISH) to map five genes, Gla, 
      G6pd, Hprt, Pgk1 and Xist, to two of the largest conserved segments of X material 
      in five members of the genus Microtus (grey vole) and show that vole X 
      chromosomes demonstrate greater homology to human than to mouse. Cytogenetic 
      analysis indicates a relatively high frequency of rearrangement during vole 
      evolution, although certain blocks of homology appear to be highly conserved in 
      all species studied to date. On this basis we were able to predict the probable 
      location of the rat X inactivation centre (Xic) based solely on high-resolution 
      G-banding. Our prediction was then confirmed by mapping the rat Xist gene by 
      FISH. The possible significance of conserving long-range chromosome structure in 
      the vicinity of the Xic is discussed with respect to the mechanism of X 
      inactivation.
FAU - Nesterova, T B
AU  - Nesterova TB
AD  - MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith 
      Hospital, London, UK.
FAU - Duthie, S M
AU  - Duthie SM
FAU - Mazurok, N A
AU  - Mazurok NA
FAU - Isaenko, A A
AU  - Isaenko AA
FAU - Rubtsova, N V
AU  - Rubtsova NV
FAU - Zakian, S M
AU  - Zakian SM
FAU - Brockdorff, N
AU  - Brockdorff N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (Transcription Factors)
RN  - 0 (XIST non-coding RNA)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
RN  - EC 2.7.2.3 (Phosphoglycerate Kinase)
RN  - EC 3.2.1.22 (alpha-Galactosidase)
SB  - IM
MH  - Animals
MH  - Arvicolinae/*genetics
MH  - Cells, Cultured
MH  - *Chromosome Mapping
MH  - DNA Probes
MH  - Dosage Compensation, Genetic
MH  - Evolution, Molecular
MH  - Fibroblasts
MH  - Gene Rearrangement
MH  - Genetic Linkage
MH  - Glucosephosphate Dehydrogenase/genetics
MH  - Hypoxanthine Phosphoribosyltransferase/genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Phosphoglycerate Kinase/genetics
MH  - RNA, Long Noncoding
MH  - *RNA, Untranslated
MH  - Rats
MH  - Sequence Homology, Nucleic Acid
MH  - Transcription Factors/genetics
MH  - X Chromosome/*genetics
MH  - alpha-Galactosidase/genetics
EDAT- 1998/03/24 00:00
MHDA- 1998/03/24 00:01
CRDT- 1998/03/24 00:00
PHST- 1998/03/24 00:00 [pubmed]
PHST- 1998/03/24 00:01 [medline]
PHST- 1998/03/24 00:00 [entrez]
AID - 10.1023/a:1009266324602 [doi]
PST - ppublish
SO  - Chromosome Res. 1998 Jan;6(1):41-8. doi: 10.1023/a:1009266324602.