PMID- 9512326
OWN - NLM
STAT- MEDLINE
DCOM- 19980324
LR  - 20041117
IS  - 0001-6349 (Print)
IS  - 0001-6349 (Linking)
VI  - 77
IP  - 2
DP  - 1998 Feb
TI  - Incidence of sex chromosome abnormalities in spermatozoa from patients entering 
      an IVF or ICSI protocol.
PG  - 191-7
AB  - OBJECTIVE: The objective of this study was the determination of sex chromosome 
      aneuploidy frequency in spermatozoa from patients included in an in vitro 
      fertilization (IVF) or intra cytoplasmic sperm injection (ICSI) protocol. 
      METHODS: Spermatozoa from nineteen patients, including patients with normal 
      seminal parameters according to World Health Organization (WHO) criteria and 
      patients exhibiting abnormal seminal parameters, were analyzed by dual color 
      fluorescence in situ hybridization (FISH) for aneuploidies of the X and Y 
      chromosomes. Our technique, using only probes for sex chromosomes and not for 
      autosomes, does not discriminate between hyperhaploid and diploid sperm nuclei. 
      The results were analyzed in two different ways: in relation to the semen status, 
      denoted normal or abnormal and with regard to the ability of the sperm to 
      fertilize oocytes when IVF or ICSI was performed. RESULTS: Abnormal semen showed 
      a significant increase in the overall rate of sperm nuclei with XY, XX and YY sex 
      chromosome complements, 1.59% compared to normal semen, 0.78% (p<0.02). Semen 
      shown to be able to fertilize oocytes only by ICSI showed a higher incidence of 
      XY-bearing spermatozoa, 1.26%, compared to semen able to fertilize oocytes by 
      conventional IVF, 0.37% (p<0.001). The incidence of XX-or YY-bearing sperm nuclei 
      was also significantly elevated in the ICSI group (0.25% XX, 0.50% YY) (p<0.02) 
      as compared to the IVF group (0.06% XX, 0.16% YY). CONCLUSIONS: We concluded that 
      infertile men requiring ICSI treatment showed a higher incidence of sex 
      chromosome aneuploidy, due to meiosis I and II nondisjunction, in their 
      spermatozoa as compared to men requiring IVF for reasons of predominantly female 
      infertility.
FAU - Storeng, R T
AU  - Storeng RT
AD  - Laboratoire de FIV et Biologie de la Reproduction, Hopital Necker, Sevres, 
      France.
FAU - Plachot, M
AU  - Plachot M
FAU - Theophile, D
AU  - Theophile D
FAU - Mandelbaum, J
AU  - Mandelbaum J
FAU - Belaisch-Allart, J
AU  - Belaisch-Allart J
FAU - Vekemans, M
AU  - Vekemans M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Acta Obstet Gynecol Scand
JT  - Acta obstetricia et gynecologica Scandinavica
JID - 0370343
SB  - IM
MH  - *Aneuploidy
MH  - Female
MH  - *Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Female
MH  - Infertility, Male
MH  - Male
MH  - Semen
MH  - *Sex Chromosome Aberrations
MH  - Spermatozoa/abnormalities
MH  - X Chromosome/*genetics
MH  - Y Chromosome/*genetics
EDAT- 1998/03/25 00:00
MHDA- 1998/03/25 00:01
CRDT- 1998/03/25 00:00
PHST- 1998/03/25 00:00 [pubmed]
PHST- 1998/03/25 00:01 [medline]
PHST- 1998/03/25 00:00 [entrez]
PST - ppublish
SO  - Acta Obstet Gynecol Scand. 1998 Feb;77(2):191-7.