PMID- 9514408
OWN - NLM
STAT- MEDLINE
DCOM- 19980402
LR  - 20190831
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 18
IP  - 3
DP  - 1998 Mar
TI  - Human vascular smooth muscle cells express receptors for CC chemokines.
PG  - 397-403
AB  - Arteriosclerotic lesions are characterized by the accumulation of T lymphocytes 
      and monocytes and the proliferation of intimal smooth muscle cells. Expression of 
      the chemokine monocyte chemoattractant protein-1 (MCP- 1) has been observed in 
      arteriosclerotic plaques and has been proposed to mediate the transendothelial 
      migration of mononuclear cells. More recently, MCP-1 has been proposed to affect 
      the proliferation and migration of vascular smooth muscle cells (VSMCs). We have 
      used reverse transcription-polymerase chain reaction (RT-PCR) to investigate 
      chemokine mRNA expression in human arteriosclerotic lesions obtained from 
      surgical biopsy of diseased vascular tissue and show, in addition to MCP-1, 
      expression of the chemokine macrophage inflammatory protein-1alpha (MIP-1alpha) 
      at higher levels than in "normal" aortic tissue. We have also used RT-PCR to 
      characterize the expression of known chemokine receptors by primary human VSMCs. 
      Messenger RNA for the MIP-1alpha/RANTES receptor, CCR-1, and the MCP-1/MCP-3 
      receptor, CCR-2, was expressed by unstimulated VSMCs grown under serum-free 
      culture conditions for 24 hours. The receptors CCR-3, CCR-4, CCR-5, CXCR-1, and 
      CXCR-2 were not expressed by VSMCs. The presence of functionally coupled 
      receptors for MIP-1alpha on VSMCs was demonstrated by specific binding of 
      biotinylated MIP-1alpha and increases in intracellular Ca2+ levels after exposure 
      to this chemokine. Taken together, these results suggest that chemokines are 
      likely to be involved in arteriosclerosis and may play a role in modulating the 
      function of VSMCs in vivo.
FAU - Hayes, I M
AU  - Hayes IM
AD  - Rhone Poulenc-Rorer, Dagenham Research Centre, Discovery Biology, Essex, UK.
FAU - Jordan, N J
AU  - Jordan NJ
FAU - Towers, S
AU  - Towers S
FAU - Smith, G
AU  - Smith G
FAU - Paterson, J R
AU  - Paterson JR
FAU - Earnshaw, J J
AU  - Earnshaw JJ
FAU - Roach, A G
AU  - Roach AG
FAU - Westwick, J
AU  - Westwick J
FAU - Williams, R J
AU  - Williams RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Arteriosclerosis/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5/metabolism
MH  - Female
MH  - Humans
MH  - Macrophage Inflammatory Proteins/metabolism
MH  - Male
MH  - Muscle, Smooth, Vascular/cytology/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/metabolism
MH  - Receptors, Chemokine/genetics/*metabolism
MH  - Transcription, Genetic
EDAT- 1998/03/26 00:00
MHDA- 1998/03/26 00:01
CRDT- 1998/03/26 00:00
PHST- 1998/03/26 00:00 [pubmed]
PHST- 1998/03/26 00:01 [medline]
PHST- 1998/03/26 00:00 [entrez]
AID - 10.1161/01.atv.18.3.397 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 1998 Mar;18(3):397-403. doi: 
      10.1161/01.atv.18.3.397.