PMID- 9514962
OWN - NLM
STAT- MEDLINE
DCOM- 19980507
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 242
IP  - 2
DP  - 1998 Mar 15
TI  - Nucleolar localization of mouse mammary tumor virus proteins in T-cell lymphomas.
PG  - 246-54
AB  - To characterize novel proteins expressed in lymphoma cells, monoclonal antibodies 
      (MAbs) were raised against variant S49 mouse lymphoma cells. Immunoperoxidase 
      analysis with a specific MAb, named M-66, revealed nuclear localization with 
      prominent staining in the nucleoli of both tumorigenic (T-63) cells and 
      nontumorigenic, immunogenic (T-25-Adh) cells. Weak signals were also observed in 
      the cytoplasm and plasma membrane of both cells. Western blot analysis with M-66 
      antibody revealed a 14-kDa protein in nuclear extracts of both T-25-Adh and T-63 
      cells. An additional nuclear 21-kDa protein was evident only in T-63 cells. M-66 
      identified several clones from a T-25-Adh cDNA expression library. These clones 
      demonstrated extensive homology (approximately 95% identity throughout their 
      length) to the mouse mammary tumor virus (MMTV) env and LTR regions. Extensive 
      amino acid sequence homology (approximately 90% identity) between the clones and 
      the env protein was observed. M-66 identified the 14-kDa protein in another MMTV 
      bearing T-cell lymphoma, EL-4. Immunoperoxidase analysis of EL-4 cells with M-66 
      also revealed prominent nucleolar staining. MMTV-negative cells and MMTV-positive 
      cells of nonlymphocytic origin were devoid of both 14- and 21-kDa proteins. 
      Moreover, an anti-MMTV gp52 (env) antibody precipitated the 21-kDa protein in 
      T-63 cells. We thus suggest that MMTV bearing T-cell lymphomas express nucleolar 
      proteins translated from the env region of MMTV.
FAU - Hoch-Marchaim, H
AU  - Hoch-Marchaim H
AD  - Department of Cell and Animal Biology, Hebrew University of Jerusalem, Israel.
FAU - Hasson, T
AU  - Hasson T
FAU - Rorman, E
AU  - Rorman E
FAU - Cohen, S
AU  - Cohen S
FAU - Hochman, J
AU  - Hochman J
LA  - eng
SI  - GENBANK/AF043688
SI  - GENBANK/AF043689
SI  - GENBANK/AF043690
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigens, Viral, Tumor/*metabolism
MH  - Blotting, Western
MH  - Cell Membrane/metabolism
MH  - Cell Nucleolus/*metabolism/virology
MH  - Cytoplasm/metabolism
MH  - DNA, Neoplasm/analysis
MH  - Gene Library
MH  - Immunoenzyme Techniques
MH  - Lymphoma, T-Cell/*metabolism/ultrastructure/*virology
MH  - Mammary Tumor Virus, Mouse/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Immunoelectron
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*metabolism
MH  - Precipitin Tests
MH  - Protein Biosynthesis
MH  - Sequence Homology, Amino Acid
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
MH  - Viral Envelope Proteins/genetics/*metabolism
EDAT- 1998/03/26 00:00
MHDA- 1998/03/26 00:01
CRDT- 1998/03/26 00:00
PHST- 1998/03/26 00:00 [pubmed]
PHST- 1998/03/26 00:01 [medline]
PHST- 1998/03/26 00:00 [entrez]
AID - S0042-6822(97)98997-2 [pii]
AID - 10.1006/viro.1997.8997 [doi]
PST - ppublish
SO  - Virology. 1998 Mar 15;242(2):246-54. doi: 10.1006/viro.1997.8997.