PMID- 9517566
OWN - NLM
STAT- MEDLINE
DCOM- 19980505
LR  - 20191102
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 22
IP  - 4
DP  - 1998 Apr
TI  - Enhanced GFAP expression in astrocytes of transgenic mice expressing the human 
      brain-specific trypsinogen IV.
PG  - 338-47
AB  - We recently identified a cDNA encoding a human brain specific trypsinogen 
      (trypsinogen IV). In order to test whether trypsinogen IV is involved in CNS 
      diseases of, or injury response in, mammalian brain, a mouse model was developed 
      in which the human trypsinogen IV was expressed specifically in neurons. 
      Immunocytochemical analysis of the brains of transgenic mice revealed a striking 
      enhancement of glial fibrillar acidic protein (GFAP) expression in astrocytes. 
      This remarkable astrocytic reaction was detected in the brains of mice as young 
      as 2 months and did not diminish in the older animals we tested. However, we did 
      not find gross evidence for neurodegeneration, nor for reactive microglial cells. 
      The long-term survival of these animals should provide a model with which to 
      study the mechanism of nerve-astroglia interactions. In addition, the possible 
      participation of trypsin IV in the metabolism of the Alzheimer precursor protein 
      (APP) was investigated by immunostaining brains from transgenic mice with 
      beta-amyloid (betaA4) antibodies. Immunocytochemical staining of brains from one 
      year old transgenic mice revealed an intense intracellular betaA4-like signal in 
      neurons.
FAU - Minn, A
AU  - Minn A
AD  - Institut fur Genetik, Lindenthal, Koln, Germany.
FAU - Schubert, M
AU  - Schubert M
FAU - Neiss, W F
AU  - Neiss WF
FAU - Muller-Hill, B
AU  - Muller-Hill B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (RNA, Messenger)
RN  - 9002-08-8 (Trypsinogen)
SB  - IM
MH  - Amyloid beta-Peptides/biosynthesis
MH  - Animals
MH  - Antibody Specificity
MH  - Astrocytes/*metabolism
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Brain/*enzymology
MH  - Female
MH  - Glial Fibrillary Acidic Protein/*biosynthesis
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/biosynthesis
MH  - Trypsinogen/*biosynthesis
MH  - Up-Regulation
EDAT- 1998/03/28 03:17
MHDA- 2000/06/20 09:00
CRDT- 1998/03/28 03:17
PHST- 1998/03/28 03:17 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/03/28 03:17 [entrez]
AID - 10.1002/(SICI)1098-1136(199804)22:4<338::AID-GLIA3>3.0.CO;2-# [pii]
AID - 10.1002/(sici)1098-1136(199804)22:4<338::aid-glia3>3.0.co;2-# [doi]
PST - ppublish
SO  - Glia. 1998 Apr;22(4):338-47. doi: 
      10.1002/(sici)1098-1136(199804)22:4<338::aid-glia3>3.0.co;2-#.