PMID- 9518269
OWN - NLM
STAT- MEDLINE
DCOM- 19980326
LR  - 20090929
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 391
IP  - 2
DP  - 1998 Feb 9
TI  - Retinoid X receptor gamma gene transcripts are expressed by a subset of early 
      generated retinal cells and eventually restricted to photoreceptors.
PG  - 204-13
AB  - We have examined the distribution of the retinoid X receptor gamma (RXRgamma) in 
      the developing chicken retina by using in situ hybridization and RNase protection 
      assays. We detected RXRgamma transcripts as early as 4 days of embryonic 
      development (d4) in central regions of the retina, spreading to more peripheral 
      regions by d8. The first few RXRgamma-positive cells were scattered within the 
      depth of the retinal neuroepithelium, but as they increased in number they became 
      localized predominantly to the apical (outer, ventricular) layer. The identity of 
      the RXRgamma-positive cells at these stages is unknown, due to the lack of cell 
      type-specific markers. By d10, when photoreceptors and ganglion cells have been 
      generated and begun to establish their definitive layers, RXRgamma-positive cells 
      were virtually restricted to the photoreceptor layer, and maintained this 
      distribution to posthatch stages. RNase protection assays were performed on whole 
      retinae to verify the temporal pattern of in situ hybridization results and 
      showed that between d5 and d16 there was a significant increase in the mRNA 
      levels of the RXRgamma2 isoform. Between d16 and early posthatch stages the level 
      of RXRgamma2 mRNA did not change significantly. Consistent with previous studies, 
      mRNA levels of the RXRgamma1 isoform were substantially lower than mRNA levels of 
      the RXRgamma2 isoform at all time points examined. These results demonstrate that 
      RXRgamma mRNA is expressed in photoreceptors in the developing chicken retina and 
      implicate RXRgamma as the earliest marker of photoreceptor differentiation 
      documented to date.
FAU - Hoover, F
AU  - Hoover F
AD  - Department of Anatomy, University of Oslo, Norway.
FAU - Seleiro, E A
AU  - Seleiro EA
FAU - Kielland, A
AU  - Kielland A
FAU - Brickell, P M
AU  - Brickell PM
FAU - Glover, J C
AU  - Glover JC
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Chick Embryo
MH  - Chickens
MH  - In Situ Hybridization
MH  - Photoreceptor Cells/embryology/growth & development/*metabolism
MH  - RNA, Messenger/*biosynthesis
MH  - Receptors, Retinoic Acid/*genetics
MH  - Retinal Ganglion Cells/*metabolism
MH  - Retinoid X Receptors
MH  - Transcription Factors/*genetics
EDAT- 1998/03/28 03:18
MHDA- 2000/06/20 09:00
CRDT- 1998/03/28 03:18
PHST- 1998/03/28 03:18 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/03/28 03:18 [entrez]
AID - 10.1002/(SICI)1096-9861(19980209)391:2<204::AID-CNE4>3.0.CO;2-6 [pii]
PST - ppublish
SO  - J Comp Neurol. 1998 Feb 9;391(2):204-13.