PMID- 9520301
OWN - NLM
STAT- MEDLINE
DCOM- 19980402
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 210
IP  - 2
DP  - 1997 Dec 29
TI  - A method for production and determination of histamine releasing activity from 
      human peripheral blood mononuclear cells.
PG  - 185-93
AB  - Histamine releasing factors, i.e. cytokines capable of inducing histamine release 
      from basophils or mast cells, have been suggested to be involved in the 
      pathogenesis of, for example, allergic late-phase reactions. Here we describe a 
      controlled method for production and determination of histamine releasing 
      activity (HRA) from human peripheral blood mononuclear cells (MNC). MNC were 
      incubated with concanavalin A (Con A) for 2 h and cultured for another 40 h in 
      fresh serum free medium. The culture supernatants were concentrated 19-25 fold by 
      ultrafiltration (molecular weight cut-off: 3000 Da). The preparations of HRA 
      induced dose- and Ca2+-dependent histamine release from leukocytes. Supernatants 
      of parallel cultures of unstimulated MNC did not induce histamine release. The 
      HRA was neither due to exogenous histamine releasing compounds (e.g. Con A) nor 
      to residual histamine in the preparations of HRA. The kinetics of HRA induced 
      histamine release (half-maximal release after > 40 min) were slower and more 
      protracted than those of anti-IgE induced histamine release. However, based on a 
      comparison between HRA induced histamine release from leukocytes and purified 
      (97%) basophils, this did not appear to be due to an indirect effect on the 
      basophils. Finally, neither the production of nor the response to HRA was 
      dependent on the allergic status of the donor.
FAU - Kampen, G T
AU  - Kampen GT
AD  - Laboratory of Medical Allergology, Allergy Unit, RHIMA 7542, National University 
      Hospital, Copenhagen, Denmark. kampen@post3.tele.dk
FAU - Poulsen, L K
AU  - Poulsen LK
FAU - Reimert, C M
AU  - Reimert CM
FAU - Skov, P S
AU  - Skov PS
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Chemokine CCL2)
RN  - 0 (anti-IgE antibodies)
RN  - 11028-71-0 (Concanavalin A)
SB  - IM
MH  - Adult
MH  - Antibodies, Anti-Idiotypic/immunology
MH  - Chemokine CCL2/biosynthesis
MH  - Concanavalin A/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - *Histamine Release
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Middle Aged
EDAT- 1998/03/31 00:00
MHDA- 1998/03/31 00:01
CRDT- 1998/03/31 00:00
PHST- 1998/03/31 00:00 [pubmed]
PHST- 1998/03/31 00:01 [medline]
PHST- 1998/03/31 00:00 [entrez]
AID - S0022-1759(97)00187-7 [pii]
AID - 10.1016/s0022-1759(97)00187-7 [doi]
PST - ppublish
SO  - J Immunol Methods. 1997 Dec 29;210(2):185-93. doi: 10.1016/s0022-1759(97)00187-7.