PMID- 9524311
OWN - NLM
STAT- MEDLINE
DCOM- 19980505
LR  - 20131121
IS  - 1061-186X (Print)
IS  - 1026-7158 (Linking)
VI  - 5
IP  - 1
DP  - 1997
TI  - The interaction of phospholipid liposomes with bacteria and their use in the 
      delivery of bactericides.
PG  - 25-34
AB  - Liposomes have been prepared from dipalmitoylphosphatidylcholine (DPPC) 
      incorporating the cationic lipids stearylamine (SA), dimethyldioctadecylammonium 
      bromide (DDAB) and dimethylaminoethane carbamoyl cholesterol (DCchol) and the 
      anionic lipids dipalmitoylphosphatidylglycerol (DPPG) and phosphatidylinositol 
      (PI). Their adsorption to biofilms of skin-associated bacteria (Staphylococcus 
      epidermidis and Proteus vulgaris) and oral bacteria (Streptococcus mutans and 
      sanguis) has been investigated as a function of mole % cationic and anionic 
      lipid. Targeting (adsorption) was most effective for the systems DPPC-chol-SA, 
      DPPC-DPPG and DPPC-PI liposomes to S. epidermidis. The effect of extracellular 
      mucopolysaccharide on targeting was investigated for S. epidermidis biofilms. It 
      was found that targeting increased with the level of extracellular 
      mucopolysaccharide for all liposome compositions studied. The delivery of the 
      oil-soluble bactericide Triclosan and the water soluble bactericide chlorhexidine 
      was studied for a number of liposomal compositions. Superior delivery of both 
      bactericides relative to the free bactericide occurred for DPPC-chol-SA liposomes 
      and for Triclosan delivery by DPPC-DPPG and DPPC-PI liposomes targeted to S. 
      epidermidis at low bactericide concentrations. DPPC-chol-SA liposomes were also 
      effective for delivery of Triclosan to S. sanguis biofilms. Double labelling 
      experiments using [14C]-chlorhexidine and [3H]-DPPC suggested that there was 
      exchange between adsorbed liposomes which had delivered bactericide to the 
      biofilm and those in the bulk solution implying a diffusion mechanism for 
      bactericide delivery.
FAU - Jones, M N
AU  - Jones MN
AD  - School of Biological Sciences, University of Manchester, U.K.
FAU - Song, Y H
AU  - Song YH
FAU - Kaszuba, M
AU  - Kaszuba M
FAU - Reboiras, M D
AU  - Reboiras MD
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Drug Target
JT  - Journal of drug targeting
JID - 9312476
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Anti-Infective Agents, Local)
RN  - 0 (Drug Carriers)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Liposomes)
RN  - 0 (Phospholipids)
RN  - 4NM5039Y5X (Triclosan)
RN  - R4KO0DY52L (Chlorhexidine)
SB  - IM
MH  - Anti-Bacterial Agents/*administration & dosage/*pharmacology
MH  - Anti-Infective Agents, Local/administration & dosage/pharmacology
MH  - Bacteria/*drug effects
MH  - Biofilms/drug effects
MH  - Chlorhexidine/administration & dosage/pharmacology
MH  - Drug Carriers
MH  - Glycosaminoglycans/metabolism
MH  - Liposomes/*pharmacology
MH  - Microbial Sensitivity Tests
MH  - Phospholipids/*pharmacology
MH  - Staphylococcus epidermidis/drug effects
MH  - Streptococcus mutans/drug effects
MH  - Streptococcus sanguis/drug effects
MH  - Triclosan/administration & dosage/pharmacology
EDAT- 1997/01/01 00:00
MHDA- 1998/04/03 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1998/04/03 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.3109/10611869708995855 [doi]
PST - ppublish
SO  - J Drug Target. 1997;5(1):25-34. doi: 10.3109/10611869708995855.