PMID- 9524964
OWN - NLM
STAT- MEDLINE
DCOM- 19980527
LR  - 20180213
IS  - 1021-7401 (Print)
IS  - 1021-7401 (Linking)
VI  - 4
IP  - 4
DP  - 1997 Jul-Aug
TI  - Blockade of N-methyl-D-aspartate receptor prevents hypoxic neuronal death and 
      cytokine release.
PG  - 195-9
AB  - Neuronal mortality, interleukin-1 beta (IL1 beta) and tumor necrosis factor-alpha 
      (TNF alpha) release were measured in hypoxic hippocampal neuronal cultures. 
      Release of IL 1 beta and TNF alpha was already observed in normoxic cultures, but 
      after hypoxia it was increased approximately 2-fold. Pretreatment with 
      2-amino-5-phosphonovaleric acid (APV), the N-methyl-D-aspartate (NMDA) receptor 
      antagonist, not only decreased neuronal mortality as expected, but also 
      dramatically lowered cytokine release. However, there was no relationship between 
      the neuronal mortality and the release of each cytokine both in untreated hypoxic 
      cultures and in APV-pretreated ones. We conclude that IL 1 beta and TNF alpha 
      release in hypoxia are dependent on the activation of the NMDA receptor, but that 
      this is not the main mechanism of hypoxia damage in in vitro neuronal cultures.
FAU - Di Loreto, S
AU  - Di Loreto S
AD  - Istituto di Tipizzazione Tissutale, CNR, L'Aquila, Italy. cnraq@mbox.vol.it
FAU - Balestrino, M
AU  - Balestrino M
FAU - Pellegrini, P
AU  - Pellegrini P
FAU - Berghella, A M
AU  - Berghella AM
FAU - Del Beato, T
AU  - Del Beato T
FAU - Di Marco, F
AU  - Di Marco F
FAU - Adorno, D
AU  - Adorno D
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Neuroimmunomodulation
JT  - Neuroimmunomodulation
JID - 9422763
RN  - 0 (Cytokines)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/*pharmacology
MH  - Animals
MH  - Cell Death/drug effects
MH  - Cells, Cultured
MH  - Cytokines/*metabolism
MH  - Hippocampus/*drug effects/metabolism/pathology
MH  - Hypoxia, Brain/metabolism/*prevention & control
MH  - Linear Models
MH  - Neurons/*drug effects/metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*antagonists & inhibitors
EDAT- 1997/07/01 00:00
MHDA- 1998/04/03 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1998/04/03 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1159/000097338 [doi]
PST - ppublish
SO  - Neuroimmunomodulation. 1997 Jul-Aug;4(4):195-9. doi: 10.1159/000097338.