PMID- 9525591
OWN - NLM
STAT- MEDLINE
DCOM- 19980417
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 72
IP  - 4
DP  - 1998 Apr
TI  - Immature dendritic cells selectively replicate macrophagetropic (M-tropic) human 
      immunodeficiency virus type 1, while mature cells efficiently transmit both M- 
      and T-tropic virus to T cells.
PG  - 2733-7
AB  - Dendritic cells (DCs) can develop from CD14+ peripheral blood monocytes cultured 
      in granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 
      (IL-4). By 6 days in culture, the cells have the characteristics of immature DCs 
      and can be further induced to mature by inflammatory stimuli or by 
      monocyte-conditioned medium. After infection with macrophagetropic (M-tropic) 
      human immunodeficiency virus type 1 (HIV-1), monocytes and mature DCs show a 
      block in reverse transcription and only form early transcripts that can be 
      amplified with primers for the R/U5 region. In contrast, immature DCs cultured 
      for 6 or 11 days in GM-CSF and IL-4 complete reverse transcription and show a 
      strong signal when LTR/gag primers are used. Blood monocytes and mature DCs do 
      not replicate HIV-1, whereas immature DCs can be productively infected, but only 
      with M-tropic HIV-1. The virus produced by immature DCs readily infects activated 
      T cells. Although mature DCs do not produce virus, these cells transmit both M- 
      and T-tropic virus to T cells. In the cocultures, both DCs and T cells must 
      express functional chemokine coreceptors for viral replication to occur. 
      Therefore, the developmental stage of DCs can influence the interaction of these 
      cells with HIV-1 and influence the extent to which M-tropic and T-tropic virus 
      can replicate.
FAU - Granelli-Piperno, A
AU  - Granelli-Piperno A
AD  - Laboratory of Cellular Physiology and Immunology, Rockefeller University, New 
      York, New York 10021, USA. piperno@rockvax.rockefeller.edu
FAU - Delgado, E
AU  - Delgado E
FAU - Finkel, V
AU  - Finkel V
FAU - Paxton, W
AU  - Paxton W
FAU - Steinman, R M
AU  - Steinman RM
LA  - eng
GR  - N01AI40045/AI/NIAID NIH HHS/United States
GR  - R01 AI040874/AI/NIAID NIH HHS/United States
GR  - AI 40874/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Receptors, CCR5)
SB  - IM
MH  - Cell Communication
MH  - Cells, Cultured
MH  - Dendritic Cells/physiology/*virology
MH  - HIV-1/*physiology
MH  - Humans
MH  - Macrophages/*virology
MH  - Receptors, CCR5/metabolism
MH  - T-Lymphocytes/*virology
MH  - *Virus Replication
PMC - PMC109716
EDAT- 1998/04/03 00:00
MHDA- 1998/04/03 00:01
PMCR- 1998/04/01
CRDT- 1998/04/03 00:00
PHST- 1998/04/03 00:00 [pubmed]
PHST- 1998/04/03 00:01 [medline]
PHST- 1998/04/03 00:00 [entrez]
PHST- 1998/04/01 00:00 [pmc-release]
AID - 1215 [pii]
AID - 10.1128/JVI.72.4.2733-2737.1998 [doi]
PST - ppublish
SO  - J Virol. 1998 Apr;72(4):2733-7. doi: 10.1128/JVI.72.4.2733-2737.1998.