PMID- 9525671
OWN - NLM
STAT- MEDLINE
DCOM- 19980417
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 72
IP  - 4
DP  - 1998 Apr
TI  - Mutational analysis of the rous sarcoma virus DR posttranscriptional control 
      element.
PG  - 3407-11
AB  - The direct repeat (DR) sequences flanking the src gene in Rous sarcoma virus are 
      essential posttranscriptional control elements; at least one copy of this 
      sequence is necessary for cytoplasmic accumulation of unspliced viral RNA. These 
      sequences promote Rev-independent human immunodeficiency virus type 1 expression, 
      suggesting they act as constitutive transport elements (CTEs). To determine which 
      regions of this sequence are critical for CTE function, mutations in the 
      downstream DR were generated and tested in a viral deletion construct lacking src 
      and the upstream DR. Two single-point mutations and three different clustered 
      mutations caused substantial reductions in reverse transcriptase activity, Gag 
      protein levels, and unspliced viral RNA in the cytoplasm. Three conserved regions 
      of the CTE, including nucleotides 8844 to 8847, 8862 to 8864, and 8868 to 8870, 
      were most sensitive to inactivation by mutagenesis.
FAU - Ogert, R A
AU  - Ogert RA
AD  - Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
FAU - Beemon, K L
AU  - Beemon KL
LA  - eng
GR  - R56 CA048746/CA/NCI NIH HHS/United States
GR  - R01 CA048746/CA/NCI NIH HHS/United States
GR  - CA48746/CA/NCI NIH HHS/United States
GR  - GM48327/GM/NIGMS NIH HHS/United States
GR  - 52T32G07231/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Gene Products, gag)
RN  - 0 (RNA, Viral)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
SB  - IM
MH  - Animals
MH  - Avian Sarcoma Viruses/*genetics
MH  - Base Sequence
MH  - Cytoplasm/metabolism
MH  - DNA Mutational Analysis
MH  - Gene Products, gag/biosynthesis
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Point Mutation
MH  - RNA Splicing
MH  - *RNA, Viral
MH  - RNA-Directed DNA Polymerase/metabolism
MH  - *Regulatory Sequences, Nucleic Acid
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Virion
PMC - PMC109836
EDAT- 1998/04/03 00:00
MHDA- 1998/04/03 00:01
PMCR- 1998/04/01
CRDT- 1998/04/03 00:00
PHST- 1998/04/03 00:00 [pubmed]
PHST- 1998/04/03 00:01 [medline]
PHST- 1998/04/03 00:00 [entrez]
PHST- 1998/04/01 00:00 [pmc-release]
AID - 1278 [pii]
AID - 10.1128/JVI.72.4.3407-3411.1998 [doi]
PST - ppublish
SO  - J Virol. 1998 Apr;72(4):3407-11. doi: 10.1128/JVI.72.4.3407-3411.1998.