PMID- 9526545
OWN - NLM
STAT- MEDLINE
DCOM- 19980421
LR  - 20200304
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 141
IP  - 12
DP  - 1996
TI  - Physical and genetic maps of the human herpesvirus 7 strain SB genome.
PG  - 2387-408
AB  - Human herpesvirus 7 (HHV-7) is a close relative of human herpesvirus 6A (HHV-6A) 
      and human herpesvirus 6B (HHV-6B) based on limited biologic and genetic data. In 
      this work we describe physical and genetic maps for HHV-7 strain SB [HHV-7(SB)], 
      which was obtained from the saliva of a healthy adult. The HHV-7(SB) genome 
      length is approximately 144 kb by clamped homogeneous electric field gel 
      electrophoresis and approximately 135 kb by summation of restriction endonuclease 
      fragments. We constructed plasmid clones and PCR amplimers that span the HHV-7 
      genome, except for the genomic termini, and determined the maps of the 
      restriction endonuclease cleavage sites for BamHI, PstI, and SacI. The HHV-7(SB) 
      genome is composed of a single unique region of approximately 122 kb bounded at 
      each end by a 6 kb direct repeat. Homologs to thirty-five herpesvirus genes were 
      identified. The highest similarity was with the HHV-6 genes, with an average 
      amino acid identity of 50%, followed by the human cytomegalovirus counterpart. 
      The genomic and genetic maps indicated that the HHV-7 and HHV-6 genomes are 
      colinear. There was no sequence variation in a segment of the gene encoding the 
      DNA polymerase-associated factor homolog among six HHV-7 isolates, while the 
      corresponding segment of the HHV-6A and HHV-6B counterparts differed by 4.6%. 
      These data support previous observations that the closest genetic relatives of 
      HHV-7 are betaherpesviruses.
FAU - Dominguez, G
AU  - Dominguez G
AD  - Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
FAU - Black, J B
AU  - Black JB
FAU - Stamey, F R
AU  - Stamey FR
FAU - Inoue, N
AU  - Inoue N
FAU - Pellett, P E
AU  - Pellett PE
LA  - eng
PT  - Journal Article
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Cytomegalovirus/genetics
MH  - DNA Primers
MH  - DNA, Viral/analysis/*genetics
MH  - DNA-Directed DNA Polymerase/genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Gene Library
MH  - Genes, Viral
MH  - Genome, Viral
MH  - Herpesvirus 6, Human/genetics
MH  - Herpesvirus 7, Human/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Phylogeny
MH  - Plasmids
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 1996/01/01 00:00
MHDA- 1998/04/04 00:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 1998/04/04 00:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1007/BF01718639 [doi]
PST - ppublish
SO  - Arch Virol. 1996;141(12):2387-408. doi: 10.1007/BF01718639.