PMID- 9526660
OWN - NLM
STAT- MEDLINE
DCOM- 19980520
LR  - 20180928
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 24
IP  - 3
DP  - 1998 Mar
TI  - Localization of trinucleotide repeat sequences in myotonic dystrophy cells using 
      a single fluorochrome-labeled PNA probe.
PG  - 472-6
AB  - A labeled peptide nucleic acid (PNA) antisense probe was used to study the 
      spatial distribution of triplet repeats (CTG) in human myotonic dystrophy (DM) 
      cells by high-resolution fluorescence in situ hybridization (FISH). It was found 
      that transcripts containing triplet repeats were present as a number of discrete 
      foci in the DM nuclei. Greater numbers of foci were visible with the PNA probe 
      than a comparable DNA probe. The PNA probe was also used to visualize the 
      triplet-repeat expansion within the DM gene located on chromosome 19. Using the 
      intensity of the expanded triplet-repeat on the chromosomes as a reference, it 
      was estimated there were between 15-230 RNA molecules in each focus observed in 
      DM nuclei.
FAU - Taneja, K L
AU  - Taneja KL
AD  - University of Massachusetts Medical Center, Worcester, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Peptides)
SB  - IM
MH  - Cell Nucleus/chemistry/genetics/pathology
MH  - Chromosomes, Human/chemistry
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Myotonic Dystrophy/*genetics/pathology
MH  - Oligodeoxyribonucleotides/metabolism
MH  - Oligonucleotide Probes/metabolism
MH  - Oligonucleotides, Antisense/metabolism
MH  - Peptides/genetics
MH  - Trinucleotide Repeats/*genetics
EDAT- 1998/04/04 00:00
MHDA- 1998/04/04 00:01
CRDT- 1998/04/04 00:00
PHST- 1998/04/04 00:00 [pubmed]
PHST- 1998/04/04 00:01 [medline]
PHST- 1998/04/04 00:00 [entrez]
AID - 10.2144/98243rr02 [doi]
PST - ppublish
SO  - Biotechniques. 1998 Mar;24(3):472-6. doi: 10.2144/98243rr02.