PMID- 9526701
OWN - NLM
STAT- MEDLINE
DCOM- 19980429
LR  - 20191102
VI  - 14
IP  - 3
DP  - 1998 Jan
TI  - Application of nested PCR and mass spectrometry for DNA-based virus detection: 
      HBV-DNA detected in the majority of isolated anti-HBc positive sera.
PG  - 97-102
AB  - DNA preparations from three different groups of serum samples were examined for 
      HBV-DNA via a nested polymerase chain reaction assay (lower detection limit: 10 
      viral genomes in 100 microliters serum): Group I consisted of 11 uninfected 
      control sera, group II consisted of sera obtained from 11 HBV infected patients 
      and group III consisted of 21 isolated anti-HBc positive samples. The 21 samples 
      from group III were HBV-DNA negative according to a conventional non-nested PCR 
      assay and hybridization with a 32P-labelled probe. Using nested PCR and mass 
      spectrometry, HBV-DNA was detected in none of group I and in all of group II 
      samples. In 11 out of 21 (52%) of the isolated anti-HBc positive sera from group 
      III, HBV-DNA was detected. No correlation was observed between HBV-DNA positivity 
      and anti-HBc titers. Matrix-assisted laser desorption/ionization time-of-flight 
      (MALDI-TOF) mass spectrometry provided a fast, sensitive and non-radioactive 
      assay for the detection of PCR products without the need for gel electrophoresis 
      or hybridization with labelled probes.
FAU - Jurinke, C
AU  - Jurinke C
AD  - Department of Biochemistry and Molecular Biology, Faculty of Chemistry, 
      University of Hamburg, Germany.
FAU - Zollner, B
AU  - Zollner B
FAU - Feucht, H H
AU  - Feucht HH
FAU - van den Boom, D
AU  - van den Boom D
FAU - Jacob, A
AU  - Jacob A
FAU - Polywka, S
AU  - Polywka S
FAU - Laufs, R
AU  - Laufs R
FAU - Koster, H
AU  - Koster H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Genet Anal
JT  - Genetic analysis : biomolecular engineering
JID - 9509403
RN  - 0 (DNA, Viral)
RN  - 0 (Hepatitis B Antibodies)
RN  - 0 (Hepatitis B Core Antigens)
SB  - IM
MH  - DNA, Viral/*blood
MH  - Hepatitis B/virology
MH  - Hepatitis B Antibodies/*blood
MH  - Hepatitis B Core Antigens/immunology
MH  - Hepatitis B virus/genetics/*isolation & purification
MH  - Humans
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 1998/04/04 00:00
MHDA- 1998/04/04 00:01
CRDT- 1998/04/04 00:00
PHST- 1998/04/04 00:00 [pubmed]
PHST- 1998/04/04 00:01 [medline]
PHST- 1998/04/04 00:00 [entrez]
AID - 10.1016/s1050-3862(97)10006-7 [doi]
PST - ppublish
SO  - Genet Anal. 1998 Jan;14(3):97-102. doi: 10.1016/s1050-3862(97)10006-7.