PMID- 9527391
OWN - NLM
STAT- MEDLINE
DCOM- 19980402
LR  - 20210518
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 9
IP  - 2
DP  - 1998 Feb
TI  - Cold-induced microtubule disruption and relocalization of membrane proteins in 
      kidney epithelial cells.
PG  - 155-66
AB  - Cold preservation of kidneys is commonly used in human transplantation and in 
      vitro studies. However, although disruption of the cytoskeleton by cold has been 
      demonstrated in cultured cells, the effect of cold treatment on intact kidney is 
      poorly understood. In this study, specific antibodies were used to examine the 
      effect of hypothermia on the cytoskeletal network and the trafficking of some 
      membrane proteins in the urinary tubule. Rat kidneys were cut into thin slices 
      (approximately 0.5 mm) that were divided into several groups: (1) some were 
      immediately fixed in paraformaldehyde, sodium periodate, and lysine (PLP); (2) 
      some were stored at 4 degrees C for 15 min or 4 h before being fixed in cold PLP; 
      or (3) after 4 h cold treatment, some slices were rewarmed to 37 degrees C for 
      15, 30, and 60 min in a physiologic solution, pH 7.4, and were then fixed in warm 
      PLP. Immunofluorescence staining revealed an almost complete disruption of the 
      microtubule network in proximal tubules after 15 min cold treatment, whereas 
      microtubules in other segments were affected after 4 h. A partial recovery of the 
      microtubule network was observed after 60 min rewarming. In contrast, actin 
      filaments seemed to be resistant to cold treatment. gp330, aquaporin-2, H+ 
      ATPase, and the AE1 anion exchanger were all relocated into numerous vesicles 
      that were distributed throughout the cytoplasm after hypothermia followed by 
      rewarming, whereas Na-K-ATPase retained its basolateral localization. The 
      vasopressin-stimulated insertion of aquaporin-2 water channels into the apical 
      membrane was inhibited during the initial rewarming period after cold exposure. 
      Thus, cold preservation of tissues might impair, at least transiently, the 
      polarized membrane expression and function of some transport proteins in renal 
      epithelial cells.
FAU - Breton, S
AU  - Breton S
AD  - Renal Unit, Massachusetts General Hospital and Harvard Medical School, Boston 
      02129, USA.
FAU - Brown, D
AU  - Brown D
LA  - eng
GR  - DK 42956/DK/NIDDK NIH HHS/United States
GR  - DK38452/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Actins)
RN  - 0 (Antiporters)
RN  - 0 (Aqp2 protein, rat)
RN  - 0 (Aquaporin 2)
RN  - 0 (Aquaporin 6)
RN  - 0 (Aquaporins)
RN  - 0 (Chloride-Bicarbonate Antiporters)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Heymann Nephritis Antigenic Complex)
RN  - 0 (Ion Channels)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, LDL)
RN  - 45PG892GO1 (Evans Blue)
RN  - EC 3.6.3.10 (H(+)-K(+)-Exchanging ATPase)
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
SB  - IM
MH  - Actins/analysis/metabolism
MH  - Animals
MH  - Antiporters/analysis/metabolism
MH  - Aquaporin 2
MH  - Aquaporin 6
MH  - *Aquaporins
MH  - Chloride-Bicarbonate Antiporters
MH  - Endosomes/metabolism
MH  - Epithelial Cells/*metabolism
MH  - Evans Blue
MH  - Fluorescent Antibody Technique, Direct
MH  - Fluorescent Dyes
MH  - H(+)-K(+)-Exchanging ATPase/analysis/metabolism
MH  - Heymann Nephritis Antigenic Complex
MH  - Hypothermia, Induced/*adverse effects
MH  - Ion Channels/analysis/metabolism
MH  - Kidney/cytology/*metabolism
MH  - Kidney Tubules/cytology/metabolism
MH  - Male
MH  - Membrane Glycoproteins/analysis/*metabolism
MH  - Microscopy, Fluorescence
MH  - Microtubules/*metabolism
MH  - Proton-Translocating ATPases/analysis/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, LDL/analysis/metabolism
EDAT- 1998/04/04 00:00
MHDA- 1998/04/04 00:01
CRDT- 1998/04/04 00:00
PHST- 1998/04/04 00:00 [pubmed]
PHST- 1998/04/04 00:01 [medline]
PHST- 1998/04/04 00:00 [entrez]
AID - 10.1681/ASN.V92155 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 1998 Feb;9(2):155-66. doi: 10.1681/ASN.V92155.