PMID- 9528337
OWN - NLM
STAT- MEDLINE
DCOM- 19980519
LR  - 20131121
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 46
IP  - 2
DP  - 1998 Feb
TI  - [DNA and RNA contents of megakaryocytes using acridine orange staining].
PG  - 139-43
AB  - We developed a new microfluorometric method to measure the DNA and RNA contents 
      of individual megakaryocytes using acridine orange (AO) staining in human bone 
      marrow smears. Some bone marrow smears were fixed with ethanol and treated with 
      pretreatment solution and then stained with an AO staining solution. The DNA 
      content was assayed by measuring the green fluorescence and the RNA content was 
      assayed by measuring the red fluorescence under B excitation with 
      microfluorometer using peripheral lymphocytes as the control. The ploidy peak was 
      shown to be 16N in all of 5 normal controls, but it was 8N in the patient with 
      refractory anemia with excess of blasts in transformation (RAEB-T). There was not 
      difference in the RNA content/DNA content ratios (RI) between each ploidy in the 
      normal controls. The RI of the patient with RAEB-T was lower than that of the 
      normal controls. The measurement of the DNA-RNA contents may be useful as a new 
      megakaryocytic parameter.
FAU - Kobayashi, Y
AU  - Kobayashi Y
AD  - 1st Department of Internal Medicine, Kyoto Prefectural University of Medicine.
FAU - Takahashi, Y
AU  - Takahashi Y
FAU - Chikayama, S
AU  - Chikayama S
FAU - Ikeda, M
AU  - Ikeda M
FAU - Uoshima, N
AU  - Uoshima N
FAU - Tanaka, K
AU  - Tanaka K
FAU - Kimura, S
AU  - Kimura S
FAU - Wada, K
AU  - Wada K
FAU - Sugano, T
AU  - Sugano T
FAU - Ozawa, M
AU  - Ozawa M
FAU - Kondo, M
AU  - Kondo M
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
RN  - 0 (Coloring Agents)
RN  - 0 (Fluorescent Dyes)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Adult
MH  - Bone Marrow Cells/chemistry
MH  - *Coloring Agents
MH  - Cytophotometry/*methods
MH  - DNA/*analysis
MH  - *Fluorescent Dyes
MH  - Humans
MH  - Megakaryocytes/chemistry
MH  - Middle Aged
MH  - Ploidies
MH  - RNA/*analysis
EDAT- 1998/04/07 00:00
MHDA- 1998/04/07 00:01
CRDT- 1998/04/07 00:00
PHST- 1998/04/07 00:00 [pubmed]
PHST- 1998/04/07 00:01 [medline]
PHST- 1998/04/07 00:00 [entrez]
PST - ppublish
SO  - Rinsho Byori. 1998 Feb;46(2):139-43.