PMID- 9529055
OWN - NLM
STAT- MEDLINE
DCOM- 19980409
LR  - 20200724
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 66
IP  - 4
DP  - 1998 Apr
TI  - Characterization of a strain of Chlamydia pneumoniae isolated from a coronary 
      atheroma by analysis of the omp1 gene and biological activity in human 
      endothelial cells.
PG  - 1370-6
AB  - Chlamydia pneumoniae is a respiratory pathogen that has been associated with 
      chronic inflammatory diseases such as asthma and atherosclerosis. Recent 
      isolation of C. pneumoniae from human carotid and coronary atheromas provides 
      additional support for a role of this organism in atherogenesis. We characterized 
      the coronary strain C. pneumoniae A-03 by sequence analysis of the major outer 
      membrane protein gene (omp1). In addition, the in vitro activities of A-03 and 
      three respiratory strains of C. pneumoniae (BAL-16, TW-183, and T-2634) were 
      examined in infected human umbilical vein endothelial cells (HUVEC) by analysis 
      of the production of interleukin-8 (IL-8), monocyte chemotactic protein 1 
      (MCP-1), and soluble intercellular cell adhesion molecule 1 (sICAM-1). Sequence 
      analysis of omp1 of C. pneumoniae A-03, compared to prototype strains TW-183 and 
      AR-39, revealed five nucleotide changes resulting in nonsynonymous codons. Of 
      interest was a nonconservative amino acid substitution (Ser to Pro) in position 
      61 of variable segment 1. In vitro, the extent of MCP-1, IL-8, and sICAM-1 
      production was dependent on the C. pneumoniae strain examined at low 
      multiplicities of infection following 24 h of incubation. Strain A-03 displayed 
      the lowest stimulatory activity in infected HUVEC, while T-2634 induced the 
      highest levels of MCP-1, IL-8, and sICAM-1 among all strains examined. 
      Heat-inactivated C. pneumoniae failed to stimulate production of these proteins 
      by all strains tested. In contrast, only partial inhibition was observed by 
      UV-inactivated organisms. Results from this study demonstrate that unlike 
      prototype respiratory strains of C. pneumoniae, the coronary strain A-03 displays 
      divergence in the omp1 gene. In addition, the stimulation of chemokines and 
      adhesion molecules involved in the recruitment of leukocytes to sites of 
      inflammation by C. pneumoniae may be important in the pathogenesis of diseases 
      associated with this organism, including atherosclerosis.
FAU - Molestina, R E
AU  - Molestina RE
AD  - Department of Medicine, University of Louisville School of Medicine, Kentucky 
      40292, USA.
FAU - Dean, D
AU  - Dean D
FAU - Miller, R D
AU  - Miller RD
FAU - Ramirez, J A
AU  - Ramirez JA
FAU - Summersgill, J T
AU  - Summersgill JT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Outer Membrane Proteins/*genetics
MH  - Base Sequence
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Chlamydophila pneumoniae/genetics/growth & development/*physiology
MH  - Coronary Artery Disease/*microbiology
MH  - Endothelium, Vascular/cytology/microbiology/*physiology
MH  - *Genes, Bacterial
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/biosynthesis
MH  - Interleukin-8/metabolism
MH  - Molecular Sequence Data
PMC - PMC108062
EDAT- 1998/04/07 00:00
MHDA- 1998/04/07 00:01
PMCR- 1998/04/01
CRDT- 1998/04/07 00:00
PHST- 1998/04/07 00:00 [pubmed]
PHST- 1998/04/07 00:01 [medline]
PHST- 1998/04/07 00:00 [entrez]
PHST- 1998/04/01 00:00 [pmc-release]
AID - 1518 [pii]
AID - 10.1128/IAI.66.4.1370-1376.1998 [doi]
PST - ppublish
SO  - Infect Immun. 1998 Apr;66(4):1370-6. doi: 10.1128/IAI.66.4.1370-1376.1998.