PMID- 9529139
OWN - NLM
STAT- MEDLINE
DCOM- 19980416
LR  - 20190914
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 12
IP  - 3
DP  - 1998 Mar
TI  - Detection of hyperdiploid karyotypes (>50 chromosomes) in childhood acute 
      lymphoblastic leukemia (ALL) using fluorescence in situ hybridization (FISH).
PG  - 427-33
AB  - ALL patients with a hyperdiploid karyotype of more than 50 chromosomes (high 
      hyperdiploidy) carry a better prognosis in contrast to patients presenting with 
      other cytogenetic features, and an appropriate less intensive therapy protocol 
      should be developed for these patients. For this reason it is desirable to have a 
      quick screening method identifying those with this type of hyperdiploidy. We 
      therefore studied the bone marrow and/or blood cells of 278 children with ALL 
      using double target fluorescence in situ hybridization (FISH) on interphase. A 
      combination of DNA probes (repetitive, centromere specific) was applied detecting 
      chromosomes which are most frequently overrepresented in patients with 
      hyperdiploidy (>50), at chromosomes 6, 10, 17 and 18. All patients showing 
      hybridization signals differing from the normal signal distribution of two spots 
      for each tested chromosome were analyzed cytogenetically as well. 102 children 
      (102/278; 36.7%) were found to have a clone with aberrant FISH results. In 80 
      patients (80/278, 28.8%) the cytogenetic analysis detected a hyperdiploid 
      karyotype >50 chromosomes, whereas the remaining patients (n=12) could be related 
      to other ploidy subgroups, ie hyperdiploidy with 47-50 chromosomes, haploidy, 
      triploidy/tetraploidy. Comparison of the FISH results with the measurements of 
      the DNA content showed good agreement for 88.8% (208/234) of the investigated 
      patients. The detected rate of 28.8% patients with a high hyperdiploid karyotype 
      in our investigated cohort is comparable to the frequency of other studies. Only 
      one patient was not identified as having a hyperdiploid karyotype with our 
      combination of DNA probes. Our results indicate that FISH is a feasible and quick 
      screening method for the detection of hyperdiploid karyotypes (>50 chromosomes) 
      and other ploidy subgroups.
FAU - Ritterbach, J
AU  - Ritterbach J
AD  - Oncogenetic Laboratory, Children's Hospital, University of Giessen, Germany.
FAU - Hiddemann, W
AU  - Hiddemann W
FAU - Beck, J D
AU  - Beck JD
FAU - Schrappe, M
AU  - Schrappe M
FAU - Janka-Schaub, G
AU  - Janka-Schaub G
FAU - Ludwig, W D
AU  - Ludwig WD
FAU - Harbott, J
AU  - Harbott J
FAU - Lampert, F
AU  - Lampert F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Aneuploidy
MH  - Bone Marrow/pathology
MH  - Cell Nucleus/pathology
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 10
MH  - Chromosomes, Human, Pair 6
MH  - DNA Probes
MH  - Haploidy
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Infant
MH  - Interphase
MH  - Karyometry/methods
MH  - Karyotyping
MH  - Precursor Cell Lymphoblastic 
      Leukemia-Lymphoma/blood/*genetics/immunology/pathology
EDAT- 1998/04/07 00:00
MHDA- 1998/04/07 00:01
CRDT- 1998/04/07 00:00
PHST- 1998/04/07 00:00 [pubmed]
PHST- 1998/04/07 00:01 [medline]
PHST- 1998/04/07 00:00 [entrez]
AID - 10.1038/sj.leu.2400930 [doi]
PST - ppublish
SO  - Leukemia. 1998 Mar;12(3):427-33. doi: 10.1038/sj.leu.2400930.