PMID- 9530166
OWN - NLM
STAT- MEDLINE
DCOM- 19980424
LR  - 20190419
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 274
IP  - 3
DP  - 1998 Mar
TI  - Changes in pulmonary expression of hexokinase and glucose transporter mRNAs in 
      rats adapted to hyperoxia.
PG  - L320-9
LID - 10.1152/ajplung.1998.274.3.L320 [doi]
AB  - Impairment of lung aconitase activity, citric acid cycle, and mitochondrial 
      respiration by hyperoxia necessitates the elevation of glycolysis for energy 
      production and of pentose shunt activity for reducing equivalents. The molecular 
      mechanisms that allow increased glucose utilization are unknown. Adult male and 
      female rats were adapted to sublethal hyperoxia, equivalent to 83% oxygen at sea 
      level, or air for 7 days. Lung RNA and protein increased in hyperoxia (197 and 
      57%, respectively), whereas total DNA was unchanged. In hyperoxia, lung total 
      hexokinase (HK) activity increased threefold, and mRNAs for HK-II and -III were 
      specifically upregulated. HK-I mRNA was unchanged. mRNAs for HK-II and -III 
      gradually increased during the first 72 h in hyperoxia. HK-II mRNA was 
      significantly elevated at 72 h, preceding changes in lung cell populations. 
      Although virtually absent in air, HK-II activity was highly expressed in 
      hyperoxia. Among lung glucose transporters, specific expression of mRNAs for 
      GLUT-4 (insulin dependent) and sodium-glucose cotransporter-1 was decreased, 
      whereas that for GLUT-1 was minimally changed. Adaptation to hyperoxia involves 
      coordinated changes in gene expression for the proteins regulating pulmonary 
      glucose transport.
FAU - Allen, C B
AU  - Allen CB
AD  - Department of Pediatrics, National Jewish Medical and Research Center, Denver, 
      Colorado, USA.
FAU - Guo, X L
AU  - Guo XL
FAU - White, C W
AU  - White CW
LA  - eng
GR  - HL-07670/HL/NHLBI NIH HHS/United States
GR  - HL-46481/HL/NHLBI NIH HHS/United States
GR  - HL-52732/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - *Adaptation, Physiological
MH  - Animals
MH  - DNA/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Hexokinase/*biosynthesis/genetics
MH  - Lung/*enzymology
MH  - Male
MH  - Monosaccharide Transport Proteins/*biosynthesis/genetics
MH  - Oxygen/*physiology
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Up-Regulation
EDAT- 1998/04/08 00:00
MHDA- 1998/04/08 00:01
CRDT- 1998/04/08 00:00
PHST- 1998/04/08 00:00 [pubmed]
PHST- 1998/04/08 00:01 [medline]
PHST- 1998/04/08 00:00 [entrez]
AID - 10.1152/ajplung.1998.274.3.L320 [doi]
PST - ppublish
SO  - Am J Physiol. 1998 Mar;274(3):L320-9. doi: 10.1152/ajplung.1998.274.3.L320.